caa a22 4500 445802499 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2008-4 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2008-4 Simultaneous Qualitative Analysis of Coumarins in the Rhizome of Angelica polymorpha Maxim. by LC-DAD-ESI-MS [Elektronische Daten] [Na Liu, Nengjiang Yu, Jin Li, Yu Yang, Jifen Guo, Yimin Zhao] A novel method has been developed for simultaneous qualitative analysis of coumarins in the rhizome of Angelica polymorpha Maxim. with the use of high-performance liquid chromatography (LC)-photodiode array detection (DAD)-electrospray ionization (ESI)-tandem mass spectrometry (MS). LC analysis was performed on an Agilent Eclipse XDB-C18 analytical column with a gradient solvent system of water and methanol, ultraviolet (UV) absorption detection was at 300nm and peak UV spectrum and (+) ESI-MS data were acquired by DAD and a quadrupole mass spectrometer, respectively. Eight peaks were identified as linear furocoumarins including aviprin, isopimpinellin, bergapten, isooxypeucedanin, gosferol, imperatorin, phellopterin and isoimperatorin by comparing their retention time, UV, and MS spectra with that of the authentic standards, and the analysis of UV spectra and ESI-MS data of the standards gave the characteristics of UV absorption and ESI-MS fragmentation. On the basis of the above characteristics, the possible structures of four other linear furocoumarins were deduced in LC. The other chemical structures were proposed as three angular furocoumarins, a simple coumarin, two chromones, and a cinnamic acid by comparing their UV and MS data with that of literature reports. Accordingly, the method we established can simply and accurately identify the key chemical constituents in the rhizome of A. polymorpha. Springer-Verlag, 2011 Column liquid chromatography nationallicence Mass spectrometry nationallicence Ultraviolet absorption nationallicence Angelica polymorpha Maxim nationallicence Coumarins nationallicence Liu Na Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Yu Nengjiang Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Li Jin Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, People's Republic of China aut Yang Yu Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Guo Jifen Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Zhao Yimin Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1121-1129 0009-5893 73:11-12<1121 2011 73 10337 https://doi.org/10.1007/s10337-011-2008-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2008-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Na Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Yu Nengjiang Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Jin Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, People's Republic of China aut NATIONALLICENCE 700 1- Yang Yu Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Guo Jifen Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Yimin Beijing Institute of Pharmacology and Toxicology, 100850, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1121-1129 0009-5893 73:11-12<1121 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer