caa a22 4500 445802502 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2012-8 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2012-8 Immunoaffinity-Based Solid Phase Extraction for the Determination of Melamine in Animal-Derived Foods Followed by LC [Elektronische Daten] [Yong Zhong, Li Zhang, Hui Zhang, Ju Liu, Jian Wang] An immunoaffinity chromatography (IAC) column was prepared and optimized to purify melamine in animal-derived foods followed by determination with a liquid chromatography method. The column showed high binding capacity of 1,250ng for melamine with recovery higher than 96%, and the column could be renewed and repeatedly used for at least 30 cycles. The extracts of chicken, egg and milk samples were directly loaded onto IAC column for purification, and the analyte retained on the column was eluted with 4mL of modified Tris buffer without carryover. The purification effect was satisfactory and better than that of the conventional solid phase extraction. Recoveries from the melamine-fortified blank samples were between 82.5 and 101.4% with coefficient of variation (CV) lower than 10.6%. Springer-Verlag, 2011 Column liquid chromatography nationallicence Immunoaffinity chromatography nationallicence Animal-derived food nationallicence Melamine nationallicence Zhong Yong College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut Zhang Li College of Life Sciences, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut Zhang Hui College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut Liu Ju College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut Wang Jian College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1211-1215 0009-5893 73:11-12<1211 2011 73 10337 https://doi.org/10.1007/s10337-011-2012-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2012-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhong Yong College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut NATIONALLICENCE 700 1- Zhang Li College of Life Sciences, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut NATIONALLICENCE 700 1- Zhang Hui College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut NATIONALLICENCE 700 1- Liu Ju College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut NATIONALLICENCE 700 1- Wang Jian College of Animal Science and Technology, Agricultural University of Hebei, 071000, Baoding, Hebei, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1211-1215 0009-5893 73:11-12<1211 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer