caa a22 4500 445802510 CHVBK 20180317145145.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2006-6 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2006-6 LC-MS-MS Method for Quantification of Hydralazine in BALB/C Mouse Plasma and Brain: Application to Pharmacokinetic Study [Elektronische Daten] [Yanying Liu, Hui Li, Hongjun Luo, Zhexuan Lin, Wenhong Luo] A rapid, sensitive and accurate high performance liquid chromatography method using tandem mass spectrometry detection for hydralazine in BALB/C mouse plasma and brain was developed and validated. The method involved a derivatization with 2,4-pentanedione at 50°C for 1h, and a step of solid phase extraction to purify and concentrate hydralazine derivative. Chromatographic separation was carried out on an Agilent ZORBAX SB-C18 column by elution with methanol-0.01molL−1 ammonium acetate (60:40, v/v). The multiple reaction monitoring transition used for quantification was m/z 225.2→129.5 in the electrospray positive ionization mode. Good linearity was obtained over the concentration range of 10-200ngmL−1. The limits of detection were 0.49 and 1.05ngmL−1 for hydralazine in mouse plasma and brain, respectively. The limits of quantitation were 1.5 and 3.18ngmL−1 for hydralazine in mouse plasma and brain, respectively. Sample analysis time was 6min including sample separation. The method was successfully applied to a pharmacokinetic study following intraperitoneal injection of hydralazine in BALB/C mice at the dose of 20mgkg−1. Springer-Verlag, 2011 Liquid chromatography-tandem mass spectrometry nationallicence Pharmacokinetics nationallicence Hydralazine nationallicence Liu Yanying Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut Li Hui Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut Luo Hongjun Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut Lin Zhexuan Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut Luo Wenhong Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1183-1188 0009-5893 73:11-12<1183 2011 73 10337 https://doi.org/10.1007/s10337-011-2006-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2006-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Yanying Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut NATIONALLICENCE 700 1- Li Hui Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut NATIONALLICENCE 700 1- Luo Hongjun Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut NATIONALLICENCE 700 1- Lin Zhexuan Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut NATIONALLICENCE 700 1- Luo Wenhong Bioanalytical Laboratory, Shantou University Medical College, Xin Ling Road 22, 515041, Shantou, Guangdong, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1183-1188 0009-5893 73:11-12<1183 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer