caa a22 4500 44580257X CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2001-y doi (NATIONALLICENCE)springer-10.1007/s10337-011-2001-y LC-MS-MS Quantitative Determination of Brivudine in Human Plasma and Its Application to Pharmacokinetic Studies [Elektronische Daten] [Xiang-Dong Peng, Zhi-Rong Tan, Hui-Zi Wu, Gan Zhou, Chen-Xian Guo, Qi Pei, Shan Cao, Xiang-Guang Meng, Yi-Cheng Wang, Yao Chen, Dong Guo, Lan Fan, Wei Zhang, Hong-Hao Zhou] A sensitive and specific liquid chromatography-electrospray ionization-tandem mass spectrometry method has been developed and validated for the identification and quantification of brivudine in human plasma using diclofenac as an internal standard. The method involves extraction with ethyl acetate. The analyte was separated on a C18 column and analyzed in multiple reaction monitoring mode with a negative electrospray ionization interface using the [M-H]− ions, m/z 332.8→m/z 80.9 for brivudine, m/z 293.6→m/z 249.5 for diclofenac. The method was validated over the concentration range of 5.54-2,836μgL−1 for brivudine. The intra-and inter-day precisions were less than 8.91% in terms of relative standard deviation (RSD), and the accuracy was within −4.22% in terms of relative error (RE). The lower limit of quantification (LLOQ) was 5.54μgL−1 with acceptable precision and accuracy. There were almost no matrix effects. Recovery of brivudine spiked in drug-free plasma was higher than 77.17%. The method was used to study the pharmacokinetic profile of brivudine in human plasma after oral administration of brivudine tablets. Springer-Verlag, 2011 Column liquid chromatography nationallicence LC-MS-MS nationallicence Electrospray ionization nationallicence Pharmacokinetic study nationallicence Brivudine nationallicence Peng Xiang-Dong Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Tan Zhi-Rong Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Wu Hui-Zi Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Zhou Gan Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Guo Chen-Xian Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Pei Qi Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Cao Shan Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Meng Xiang-Guang Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Wang Yi-Cheng Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Chen Yao Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Guo Dong Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Fan Lan Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Zhang Wei Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Zhou Hong-Hao Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1089-1095 0009-5893 73:11-12<1089 2011 73 10337 https://doi.org/10.1007/s10337-011-2001-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2001-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Xiang-Dong Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Tan Zhi-Rong Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Wu Hui-Zi Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Gan Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Guo Chen-Xian Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Pei Qi Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Cao Shan Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Meng Xiang-Guang Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Wang Yi-Cheng Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yao Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Guo Dong Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Fan Lan Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Wei Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Hong-Hao Pharmacogenetics Research Institute, Institute of Clinical Pharmacology, Central South University, 410078, Changsha, Hunan, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1089-1095 0009-5893 73:11-12<1089 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer