caa a22 4500 445802596 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2004-8 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2004-8 Simultaneous Determination of Pregabalin, Sildenafil and Its Active Metabolite in Rat Plasma Utilising SPE Followed by LC-MS-MS [Elektronische Daten] [Paweł Dżygiel, Daniela Fraier] A sensitive and selective analytical method for the quantification of pregabalin, sildenafil and the active desmethyl metabolite of sildenafil (UK-103320) has been developed. The method can simultaneously quantify the three analytes within the expected in vivo concentration ranges using 50μL of rat plasma. It utilises solid-phase extraction followed by high performance liquid chromatography coupled with tandem mass spectrometry. Quantitation in rat plasma demonstrated good accuracy and precision over the following dynamic ranges for each analyte: pregabalin (70-10,000ngmL−1), sildenafil (1-2,000ngmL−1) and UK-103320 (1-2,000ngmL−1). For each analyte, the following lower limits of quantitation were obtained: 70ngmL−1 for pregabalin and 1ngmL−1 for sildenafil and UK-103320, respectively. The method was successfully used to analyse plasma samples from rats when pregabalin and sildenafil were administered in combination. Springer-Verlag, 2011 LC-MS-MS nationallicence Monolithic-based chromatography nationallicence Solid-phase extraction nationallicence Pregabalin nationallicence Sildenafil nationallicence Dżygiel Paweł Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, CT13 9NJ, Sandwich, Kent, UK aut Fraier Daniela Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, CT13 9NJ, Sandwich, Kent, UK aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1177-1182 0009-5893 73:11-12<1177 2011 73 10337 https://doi.org/10.1007/s10337-011-2004-8 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2004-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dżygiel Paweł Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, CT13 9NJ, Sandwich, Kent, UK aut NATIONALLICENCE 700 1- Fraier Daniela Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer Global Research and Development, CT13 9NJ, Sandwich, Kent, UK aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1177-1182 0009-5893 73:11-12<1177 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer