caa a22 4500 445802618 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2016-4 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2016-4 Development of a Stationary Phase of Vascular Smooth Muscle Cell Membrane Chromatography and Its Chromatographic Affinity Characteristics [Elektronische Daten] [Xingxin Yang, Yanli Zhang, Xiaoxia Zhang, Ruimiao Chang, Xiaoni Li] A novel stationary phase of vascular smooth muscle cell membrane chromatography (VSM-CMC) was developed by immobilizing the vascular smooth muscle cell membrane onto the surface of silica and was presented for bioaffinity chromatography. The protein level and Na+, K+-ATP enzymatic activity of the vascular smooth muscle cell membrane stationary phase (VSM-CMSP) were detected. The surface characteristics of the VSM-CMSP were tested using scanning electron microscopy and surface energy spectrometry. The retention characteristics of four dihydropyridines (amlodipine besylate, nicardipine hydrochloride, nitrendipine and nifedipine) were investigated using a VSM-CMSP column (10mm×2mm, I.D.) packed with VSM-CMSP. The logarithm of the capacity factor (logk′) was taken as a measure of the affinities of the calcium antagonists toward the vascular smooth muscle cell membrane and receptors. The surface characteristics of the VSM-CMSP were very different from that of the normal and reversed stationary phase, and the VSM-CMSP was found to have cell membrane activity and chromatographic separation. Moreover, there was a significant correlation between the affinity in the VSM-CMC system and the effect in vitro with respect to the pharmacological effect. It is concluded that the VSM-CMC system can serve as a type of bioaffinity chromatography for studying the interaction between drug molecules and target sites (e.g., receptors) in cell membranes, and for screening active compounds from complex agents. Springer-Verlag, 2011 Bioaffinity chromatography nationallicence Cell membrane chromatography nationallicence Cell membrane stationary phase nationallicence Vascular smooth muscle nationallicence Calcium antagonists nationallicence Yang Xingxin College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut Zhang Yanli College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut Zhang Xiaoxia College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut Chang Ruimiao College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut Li Xiaoni College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1065-1071 0009-5893 73:11-12<1065 2011 73 10337 https://doi.org/10.1007/s10337-011-2016-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2016-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang Xingxin College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut NATIONALLICENCE 700 1- Zhang Yanli College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut NATIONALLICENCE 700 1- Zhang Xiaoxia College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut NATIONALLICENCE 700 1- Chang Ruimiao College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut NATIONALLICENCE 700 1- Li Xiaoni College of Pharmacy, Shanxi Medical University, No 56 Xinjian South Road, 030001, Taiyuan, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1065-1071 0009-5893 73:11-12<1065 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer