caa a22 4500 445802634 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2026-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2026-2 Detection of Nandrolone and Boldenone Abuse in the Ovine by GC-MS-MS [Elektronische Daten] [Adam Clarke, James Scarth, Jonathan Hands, Philip Teale, Aileen Mill, Roy Macarthur, Jack Kay] Under European legislation, the use of anabolic steroids as growth promoters in meat production is prohibited. Currently, there is no internationally accepted method used for the detection of the potentially endogenous steroids nandrolone and boldenone in the ovine. In the current study, a multi-residue GC-MS-MS-based urinary assay has been validated for boldenone as well as the nandrolone metabolites 5α-estrane-3β,17α-diol and epinandrolone. Using a standard addition calibration line approach in pooled bovine urine, the method was linear between the endogenous concentrations and those augmented with 6,000pgmL−1. The method was then applied to populations of wether (n=242) and ewe (n=237) ovine animals in order to establish urinary thresholds for detecting nandrolone and boldenone abuse. A statistical model (the Chebyshev inequality) was used to produce threshold concentrations for each analyte. Adjustment of the nandrolone metabolite data for specific gravity, a measure of the hydration status of the animal, allowed the effective thresholds to be reduced; potentially leading to a lower number of false positives. Furthermore, the proposed epinandrolone confirmatory thresholds (38,628 and 57,950pgmL−1 in wethers and ewes, respectively) were found to be effective in detecting abuse of nandrolone for at least 1month post-dose of this steroid. However, further studies would be required to assess the efficacy of the proposed boldenone confirmatory thresholds (19,857 and 56,080pgmL−1 in wethers and ewes, respectively) since data on its excretion following administration to the ovine are lacking. Springer-Verlag, 2011 Gas chromatography-mass spectrometry nationallicence Nandrolone nationallicence Boldenone nationallicence Steroids nationallicence Ovine nationallicence Sheep nationallicence Clarke Adam HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut Scarth James HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut Hands Jonathan HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut Teale Philip HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut Mill Aileen The Food and Environment Research Agency, Sand Hutton, Y041 1LZ, York, UK aut Macarthur Roy The Food and Environment Research Agency, Sand Hutton, Y041 1LZ, York, UK aut Kay Jack Veterinary Medicines Directorate, Woodham Lane, New Haw, KT15 3LS, Addlestone, Surrey, UK aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1155-1169 0009-5893 73:11-12<1155 2011 73 10337 https://doi.org/10.1007/s10337-011-2026-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2026-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Clarke Adam HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut NATIONALLICENCE 700 1- Scarth James HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut NATIONALLICENCE 700 1- Hands Jonathan HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut NATIONALLICENCE 700 1- Teale Philip HFL Sport Science (an LGC company), Newmarket Road, CB7 5WW, Fordham, Cambridgeshire, UK aut NATIONALLICENCE 700 1- Mill Aileen The Food and Environment Research Agency, Sand Hutton, Y041 1LZ, York, UK aut NATIONALLICENCE 700 1- Macarthur Roy The Food and Environment Research Agency, Sand Hutton, Y041 1LZ, York, UK aut NATIONALLICENCE 700 1- Kay Jack Veterinary Medicines Directorate, Woodham Lane, New Haw, KT15 3LS, Addlestone, Surrey, UK aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1155-1169 0009-5893 73:11-12<1155 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer