caa a22 4500 445802650 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-1949-y doi (NATIONALLICENCE)springer-10.1007/s10337-011-1949-y Determination of Quinine and Doxycycline in Rat Plasma by LC-MS-MS: Application to a Pharmacokinetic Study [Elektronische Daten] [Liberato Brum Junior, Manoela Leal, Flávia de Toni Uchoa, Moacir Kaiser, Sílvia Guterres, Teresa Dalla Costa] A fast, sensitive, and specific LC-MS-MS method for determination of quinine (QN) and doxycycline (DOX) in rat plasma has been developed and validated. QN, DOX, and cimetidine (internal standard, IS) were extracted from the plasma by protein precipitation. The compounds were separated on a C18 column with methanol-0.1% aqueous formic acid 70:30 (v/v) as mobile phase at a flow rate of 0.5mLmin−1 (split 1:3). Detection was by positive electrospray ionization (ESI+) in multiple reaction monitoring (MRM) mode, monitoring the transitions 325.0→307.0, 445.0→428.1, and 252.8→159.0, for QN, DOX, and IS, respectively. The analysis was carried out in 2.0min and the method was linear in the plasma concentration range 5-5,000ngmL−1. The mean extraction recoveries for QN, DOX, and IS from plasma were 89.4, 90.5, and 86.3%, respectively. The method was validated for linearity, precision, accuracy, specificity, and stability; the results obtained were within the acceptable range. The proposed method was successfully applied to the determination of QN and DOX in rat plasma samples to support pharmacokinetic studies. Springer-Verlag, 2011 LC-MS-MS nationallicence Protein precipitation nationallicence Validation nationallicence Rat plasma nationallicence Pharmacokinetics nationallicence Quinine nationallicence Doxycycline nationallicence Brum Junior Liberato Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut Leal Manoela Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut de Toni Uchoa Flávia Centro Bioanalítico de Medicamentos da Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut Kaiser Moacir Centro Bioanalítico de Medicamentos da Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut Guterres Sílvia Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut Dalla Costa Teresa Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1081-1088 0009-5893 73:11-12<1081 2011 73 10337 https://doi.org/10.1007/s10337-011-1949-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1949-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Brum Junior Liberato Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Leal Manoela Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- de Toni Uchoa Flávia Centro Bioanalítico de Medicamentos da Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Kaiser Moacir Centro Bioanalítico de Medicamentos da Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Guterres Sílvia Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut NATIONALLICENCE 700 1- Dalla Costa Teresa Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga, 2752, CEP 90610-000, Porto Alegre, RS, Brazil aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1081-1088 0009-5893 73:11-12<1081 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer