caa a22 4500 445802669 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2002-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-2002-x Elucidation of the Structures of Metabolites of Picroside II in Rat Bile by LC-ESI-IT-MS [Elektronische Daten] [Tingting Li, Qiaoling Yu, Deen Han, Ying Zhao, Di Zhao, Hui Ji, Xijing Chen, Ning Li, Zhixia Qiu, Yi Zheng] Picroside II is one of the main active constituents of Picrorhiza kurroa, which has hepatoprotective, anticholestatic, antioxidant, and immune-modulating activity. To gain an understanding of the biotransformation of picroside II in vivo, liquid chromatography-electrospray ionization ion-trap mass spectrometry (LC-ESI-IT-MS) was used to investigate the metabolism of picroside II in rats after intravenous administration of a single dose. This method could simultaneously determine picroside II and its metabolites in rat bile. The bile samples were purified by use of a C18 solid-phase extraction (SPE) cartridge and were separated on a Hypersil ODS2 C18 analytical column. Two phase II metabolites of picroside II in rat bile were characterized, and elucidation of their structures was performed by comparing changes in molecular masses (ΔM), retention times, and MS2 spectral patterns of metabolites with those of the parent drug. Two metabolites identified for the first time in this research were glucuronide and sulfate conjugates. Springer-Verlag, 2011 Picroside II nationallicence Metabolism nationallicence Metabolites identification nationallicence LC-ESI-IT-MS nationallicence Li Tingting School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Yu Qiaoling School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Han Deen Department of Tradition Chinese Medicine, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Zhao Ying School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Zhao Di School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Ji Hui School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Chen Xijing School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Li Ning School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Qiu Zhixia School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Zheng Yi School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1111-1120 0009-5893 73:11-12<1111 2011 73 10337 https://doi.org/10.1007/s10337-011-2002-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2002-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Tingting School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Yu Qiaoling School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Han Deen Department of Tradition Chinese Medicine, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Ying School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Di School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Ji Hui School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xijing School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Li Ning School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Qiu Zhixia School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Yi School of Pharmacy, China Pharmaceutical University, 210009, Nanjing, Jiangsu, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1111-1120 0009-5893 73:11-12<1111 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer