caa a22 4500 445802685 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110601xx s 000 0 eng 10.1007/s10337-011-2007-5 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2007-5 Ocular Pharmacokinetic Study of l -Carnosine and N -Acetyl- l -carnosine in Rabbit by Microdialysis Coupled with UPLC-MS-MS [Elektronische Daten] [Dan Su, Yonggui Song, Lulu Geng, Binbin Wei, Pu Xie, Yue Qi, Chao Ma, Shilin Yang, Kaishun Bi, Xiaohui Chen] In order to provide useful information for rational drug design, the ocular pharmacokinetics of l-carnosine (CAR) and its acetylized prodrug N-acetyl-l-carnosine (NAC) were investigated. The in vivo microdialysis sampling coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) was developed for continuously simultaneous monitoring of CAR and NAC in rabbit aqueous humor. The measured in vitro recoveries of the probe were 61.3% for CAR and 65.8% for NAC, while in vivo recoveries decreased to 43.1% for CAR and 43.0% for NAC, respectively. The method was sensitive with LLOQ 20.5ngmL−1 for CAR and 20.4ngmL−1 for NAC. The initial data indicated that the value of C max and AUC(0-∞) of NAC were higher than these of CAR (C max 2305 vs. 1,802ngmL−1), (AUC(0-∞) 1,337 vs. 1,891ngh mL−1), which indicated that the NAC exhibited better ocular bioavailability and duration. The method was rapid, specific and sensitive for continuously monitoring of aqueous humor and it was successfully applied to pharmacokinetic studies of CAR and NAC. Springer-Verlag, 2011 Ultra-performance liquid chromatography-tandem mass spectrometry nationallicence l -carnosine nationallicence N -acetyl- l -carnosine nationallicence Microdialysis nationallicence Ocular pharmacokinetics nationallicence Su Dan The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, People's Republic of China aut Song Yonggui The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, People's Republic of China aut Geng Lulu School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Wei Binbin School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Xie Pu School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Qi Yue School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Ma Chao School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Yang Shilin The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, People's Republic of China aut Bi Kaishun School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Chen Xiaohui School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1189-1195 0009-5893 73:11-12<1189 2011 73 10337 https://doi.org/10.1007/s10337-011-2007-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2007-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Su Dan The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Song Yonggui The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Geng Lulu School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Wei Binbin School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Xie Pu School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Qi Yue School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Ma Chao School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Yang Shilin The National Pharmaceutical Engineering Center (NPEC) for Solid Preparation in Chinese Herbal Medicine, 56 Yangming Road, 330006, Nanchang, People's Republic of China aut NATIONALLICENCE 700 1- Bi Kaishun School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xiaohui School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/11-12(2011-06-01), 1189-1195 0009-5893 73:11-12<1189 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer