caa a22 4500 445802812 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10337-011-1928-3 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1928-3 Determination of Pilocarpine in Human Plasma by LC-APCI-MS-MS and Application to a Pharmacokinetic Study [Elektronische Daten] [Peng Yu, Guoping Yang, Hongyi Tan, Zeneng Cheng, Ming Song, Zhenkun Gu, Xiao Li] A sensitive, specific and rapid high performance liquid chromatography-atmospheric pressure chemical ionization source-tandem mass spectrometry (LC-APCI-MS-MS) method for the determination of pilocarpine in human plasma was developed and validated. The method is based on liquid-liquid extraction, followed by a reversed-phase liquid chromatographic separation, and detected by means of tandem mass spectrometry. The linear calibration curve covered a concentration range of 2-500μgL−1. The intra- and inter-day precisions for pilocarpine were <10% and the accuracies were between 90 and 110%. The method was applied successfully to a pharmacokinetic study involving 20 healthy Chinese male volunteers after oral administration of 6mg pilocarpine. Springer-Verlag, 2011 Column liquid chromatography nationallicence LC-APCI-MS-MS nationallicence Pilocarpine nationallicence Human plasma nationallicence Pharmacokinetic study nationallicence Yu Peng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Yang Guoping The Third Xiangya Hospital, Central South University, 410013, Changsha, China aut Tan Hongyi The Third Xiangya Hospital, Central South University, 410013, Changsha, China aut Cheng Zeneng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Song Ming School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Gu Zhenkun School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Li Xiao School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut Chromatographia Springer-Verlag 73/9-10(2011-05-01), 921-927 0009-5893 73:9-10<921 2011 73 10337 https://doi.org/10.1007/s10337-011-1928-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1928-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yu Peng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Yang Guoping The Third Xiangya Hospital, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Tan Hongyi The Third Xiangya Hospital, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Cheng Zeneng School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Song Ming School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Gu Zhenkun School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 700 1- Li Xiao School of Pharmaceutical Sciences, Central South University, 410013, Changsha, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/9-10(2011-05-01), 921-927 0009-5893 73:9-10<921 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer