caa a22 4500 445802839 CHVBK 20180317145146.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10337-011-1974-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-1974-x Development of a Parallel-Tandem Column Interface in a Two-Dimensional Liquid Chromatography System [Elektronische Daten] [Duxin Li, Lingyi Zhang, Zhicong Wang, Azeem Intisar, Weibing Zhang] An ion exchange-reversed-phase two-dimensional liquid chromatography (2D-LC) system was developed based on a newly designed interface. The interface was constructed by alternately connecting tandem columns to one of the parallel separation columns to increase the separation efficiency. Separation on the tandem column does not affect the switching frequency; the valve switching could be maintained at high frequency. The constructed interface could be used in both ambient and elevated temperature modes. Under high-temperature mode, only the tandem column was heated, which simplified the operation, decreased the backpressure, improved the solvent compatibility, and speeded up the separation. Tryptic digestion of four proteins was used to evaluate the performance of the developed system. The peak capacity of the 2D-LC system reached 650 under ambient conditions and 890 under high temperature conditions. When compared with the traditional parallel column interface constructed in this paper, the new interface produced a 60% higher peak capacity. Springer-Verlag, 2011 Two-dimensional liquid chromatography system nationallicence Ion exchange-reversed-phase chromatography nationallicence Parallel-tandem column interface nationallicence Peptides nationallicence Li Duxin Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Zhang Lingyi Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Wang Zhicong Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Intisar Azeem Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Zhang Weibing Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut Chromatographia Springer-Verlag 73/9-10(2011-05-01), 871-877 0009-5893 73:9-10<871 2011 73 10337 https://doi.org/10.1007/s10337-011-1974-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1974-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Duxin Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Lingyi Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Wang Zhicong Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Intisar Azeem Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Weibing Shanghai Key Laboratory of Functional Materials Chemistry, East China University of Science and Technology, 200237, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/9-10(2011-05-01), 871-877 0009-5893 73:9-10<871 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer