caa a22 4500 44580288X CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10337-011-1982-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-1982-x Three-Phase Solvent Bar Microextraction Combined with HPLC for Extraction and Determination of Plasma Protein Binding of Bisoprolol [Elektronische Daten] [Ming Zhang, Qing Li, Wei Ji, Shan Jiang, Chao Ma, Chao Wang, Jing Ye, Yan Cui, Wentao Liu, Kaishun Bi, Xiaohui Chen] A three-phase solvent bar microextraction (TPSBME) technique combined with high performance liquid chromatography (HPLC)-fluorescence detection was evaluated for the quantitative determination of plasma protein binding of bisoprolol. Bisoprolol was extracted from a 5.6-mL basified plasma sample (donor phase) into the organic solvent (n-octanol) impregnated in the pores of a hollow fiber and then extracted into an acidic solution (acceptor phase) inside the lumen of the hollow fiber. Metoprolol was used as the internal standard. Several parameters influencing the efficiency of the method were investigated and optimized including organic solvent (n-butanol, n-octanol, dibutyl phthalate, dihexyl ether), stirring rate (100-1,000rpm), extraction time (5-35min), extraction temperature (15-45°C), concentration of the donor phase (0.1-2M NaOH) and the acceptor phase (0.5-5M formic acid), salt concentration (2.5-10%, w/v). Under the optimal condition, extraction recoveries from plasma samples were above 61.4% for bisoprolol. The calibration curves were obtained in the range of 10-100ngmL−1 with reasonable linearity (r>0.994). The method was successfully applied to determine the plasma protein binding rate of bisoprolol. Springer-Verlag, 2011 Solvent bar microextraction nationallicence Bisoprolol nationallicence HPLC nationallicence Plasma nationallicence Zhang Ming School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Li Qing School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Ji Wei School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Jiang Shan Shenyang Greenvalley Biotechnology Industry Co. Ltd, Shenyang, People's Republic of China aut Ma Chao School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Wang Chao School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Ye Jing School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Cui Yan School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Liu Wentao School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Bi Kaishun School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Chen Xiaohui School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut Chromatographia Springer-Verlag 73/9-10(2011-05-01), 897-903 0009-5893 73:9-10<897 2011 73 10337 https://doi.org/10.1007/s10337-011-1982-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1982-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Ming School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Li Qing School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Ji Wei School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Shan Shenyang Greenvalley Biotechnology Industry Co. Ltd, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Ma Chao School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Wang Chao School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Ye Jing School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Cui Yan School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Liu Wentao School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Bi Kaishun School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 700 1- Chen Xiaohui School of Pharmacy, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/9-10(2011-05-01), 897-903 0009-5893 73:9-10<897 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer