caa a22 4500 445802901 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10337-011-1988-4 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1988-4 Epitopic Peptides Identified by LC-ELISA and LC-MS [Elektronische Daten] [Chuanqi Zhou, Qingshuang Wang] A new strategy of a liquid chromatography (LC)-enzyme linked immunosorbent assay (ELISA) combined with liquid chromatography-mass spectrometry (LC-MS) was developed to identify the epitopic peptides for the antigen in Streptococcus suis. Metallendopeptidase, a potential antigen for S. suis, was digested by trypsin. The tryptic solution was separated by LC under optimal condition and collected into tubes. After it was dried using a cooling vacuum, the immunogenecity of peptides in each tube was investigated by ELISA against its corresponding antibody. All peptides in each tube with positive signals based on ELISA results were identified by LC-MS. Accordingly, six putative peptides of metallendopeptidase were found and synthesized, all of which possessed high antigenicity recognized by the antibody. Springer-Verlag, 2011 Column liquid chromatography nationallicence ELISA nationallicence Tandem mass spectrometry nationallicence Epitopic peptide nationallicence Metallendopeptidase nationallicence Zhou Chuanqi Beijing Institute of Genomics, Chinese Academy of Sciences, No. 7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut Wang Qingshuang School of Life Science and Technology, Changchun University of Science and Technology, 7089 Weixing Road, 130022, Changchun, Jilin, People's Republic of China aut Chromatographia Springer-Verlag 73/9-10(2011-05-01), 879-887 0009-5893 73:9-10<879 2011 73 10337 https://doi.org/10.1007/s10337-011-1988-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1988-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Chuanqi Beijing Institute of Genomics, Chinese Academy of Sciences, No. 7 Beitucheng West Road, Chaoyang District, 100029, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wang Qingshuang School of Life Science and Technology, Changchun University of Science and Technology, 7089 Weixing Road, 130022, Changchun, Jilin, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/9-10(2011-05-01), 879-887 0009-5893 73:9-10<879 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer