caa a22 4500 44580291X CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10337-011-1978-6 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1978-6 LC-MS/MS Assay for Quantification of a Novel Antidiabetic Flavone Derivative S002-857 in Rat Plasma, Urine and Feces: Application in Pharmacokinetic Studies [Elektronische Daten] [Nagsen Gautam, Hari Kushwaha, Hemant Kumar, Ram Pratap, Shio Singh] This study aimed to develop an LC-MS/MS assay for the quantitation of S002-857 in plasma, urine and feces to evaluate the pharmacokinetic parameters and elimination pathway for further development of S002-857. A sensitive and selective LC-MS/MS method was developed and validated for estimating a novel antidiabetic flavone derivative, S002-857, synthesized by Central Drug Research Institute, CSIR, Lucknow, India. Plasma and urine samples were prepared by two-step liquid-liquid extraction with diethyl ether and feces by protein precipitation using acetonitrile. The analyte was chromatographed on a cyano column with methanol-ammonium acetate buffer (pH 4.6, 10mM; 90:10, v/v). The calibration curves were linear over a range of 0.78-400ngmL−1 for plasma and 1.56-400ngmL−1 for urine and feces samples. The accuracy and precision were <15% for plasma, urine and feces samples. The recoveries from spiked plasma, urine and feces samples were >85%. S002-857 was stable in plasma for 8h at ambient temperature, 30days at −60°C and after three freeze-thaw cycles. The assay was successfully applied to determine the pharmacokinetic parameters and excretion profile in male rats. Springer-Verlag, 2011 LC-MS/MS nationallicence Excretion nationallicence Pharmacokinetics nationallicence Antidiabetic nationallicence S002-857 nationallicence Gautam Nagsen Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut Kushwaha Hari Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut Kumar Hemant Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut Pratap Ram Medicinal and Process Chemistry Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut Singh Shio Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut Chromatographia Springer-Verlag 73/9-10(2011-05-01), 905-912 0009-5893 73:9-10<905 2011 73 10337 https://doi.org/10.1007/s10337-011-1978-6 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1978-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gautam Nagsen Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Kushwaha Hari Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Kumar Hemant Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Pratap Ram Medicinal and Process Chemistry Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut NATIONALLICENCE 700 1- Singh Shio Pharmacokinetics and Metabolism Division, Central Drug Research Institute, CSIR, 226001, Lucknow, India aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/9-10(2011-05-01), 905-912 0009-5893 73:9-10<905 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer