caa a22 4500 445802928 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10337-011-1993-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1993-7 Determination of Pyrroloquinoline Quinone in DNA Binding by LC [Elektronische Daten] [Xingqin Zhou, Jiankang Zhang, Xiaofeng Qin, Xindai Hu] A simple and repeatable liquid chromatography method with UV detection is described for the determination of pyrroloquinoline quinone in DNA binding studies first. Binding of pyrroloquinoline quinone to DNA was measured indirectly by determining unbound pyrroloquinoline quinone. Pyrroloquinoline quinone was chromatographed on a reversed-phase column using gradient elution with mobile phases of 0.1% trifluoroacetic acid (TFA) in deionized water and 0.1% TFA in acetonitrile at a flow-rate of 0.8mLmin−1 and detected at 249nm. The mean content of unbound pyrroloquinoline quinone to three DNA in (hydroxymethyl) aminoethane(Tris-HCl) media were dropped from 99.3 to 80.3% in 250min, and than to 64.8% at 1200min, but the binding remained constant in deionized water. The method precision were validated with RSD 1.05 and 0.69% for peak areas and retention times. Validation of the method showed that the assay was linear from 25 to 2,000μM and 5 to 2,000μM pyrroloquinoline quinone in Tris-HCl and deionized water, respectively. The accuracy for three concentrations of pyrroloquinoline quinone with different types DNA in two media were within the given range of 80.9-113.6%. Springer-Verlag, 2011 Column liquid chromatography nationallicence Binding nationallicence DNA nationallicence Pyrroloquinoline quinone nationallicence Zhou Xingqin Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, 214063, Wuxi, Jiangsu, China aut Zhang Jiankang Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, 214063, Wuxi, Jiangsu, China aut Qin Xiaofeng Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, 214063, Wuxi, Jiangsu, China aut Hu Xindai East China Normal University, 200241, Shanghai, China aut Chromatographia Springer-Verlag 73/9-10(2011-05-01), 1027-1030 0009-5893 73:9-10<1027 2011 73 10337 https://doi.org/10.1007/s10337-011-1993-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1993-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhou Xingqin Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, 214063, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Zhang Jiankang Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, 214063, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Qin Xiaofeng Key Laboratory of Nuclear Medicine, Ministry of Health, Jiangsu Key Laboratory of Molecular Nuclear Medicine, Jiangsu Institute of Nuclear Medicine, 214063, Wuxi, Jiangsu, China aut NATIONALLICENCE 700 1- Hu Xindai East China Normal University, 200241, Shanghai, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/9-10(2011-05-01), 1027-1030 0009-5893 73:9-10<1027 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer