caa a22 4500 445803002 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110501xx s 000 0 eng 10.1007/s10337-011-1985-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-1985-7 Tetrazole-Functionalized Silica for Hydrophilic Interaction Chromatography of Polar Solutes [Elektronische Daten] [Xiaojun Dai, Xiaolei Qian, Bolin Gong, Yinmao Wei] In this work, tetrazole-functionalized stationary phase was prepared with nitrile-modified silica by an ammonium-catalyzed (3+2) azide-nitrile cycloaddition reaction. The prepared stationary phase was used for separation of nucleobases and nucleosides by hydrophilic interaction chromatography (HILIC) mode. A typical HILIC mechanism was observed at higher content of acetonitrile (>85%, v/v) in the mobile phase. The retention mechanism of the column was investigated by the models used for describing partitioning and surface adsorption through adjustment ratio of water in the mobile phase, and by the influence of salt concentration, buffer pH, and temperature on the retention of solutes. The results illustrated that the surface adsorption through hydrogen bonding dominated the retention behavior of nucleobases/nucleosides under HILIC mode. From the separation ability, the tetrazole-functionalized stationary phase could become a valuable alternative for the separation of the compounds concerned. Springer-Verlag, 2011 Hydrophilic interaction chromatography nationallicence Tetrazole-functionalized silica nationallicence Nucleobases nationallicence Nucleosides nationallicence Retention mechanism nationallicence Dai Xiaojun Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, 710069, Xi'an, People's Republic of China aut Qian Xiaolei Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, 710069, Xi'an, People's Republic of China aut Gong Bolin Key Laboratory of Energy and Chemical Engineering, Ningxia University, 750021, Yinchuan, People's Republic of China aut Wei Yinmao Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, 710069, Xi'an, People's Republic of China aut Chromatographia Springer-Verlag 73/9-10(2011-05-01), 865-870 0009-5893 73:9-10<865 2011 73 10337 https://doi.org/10.1007/s10337-011-1985-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-1985-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dai Xiaojun Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, 710069, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Qian Xiaolei Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, 710069, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Gong Bolin Key Laboratory of Energy and Chemical Engineering, Ningxia University, 750021, Yinchuan, People's Republic of China aut NATIONALLICENCE 700 1- Wei Yinmao Key Laboratory of Synthetic and Natural Function Molecule Chemistry of Ministry of Education, College of Chemistry and Material Science, Northwest University, 710069, Xi'an, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 73/9-10(2011-05-01), 865-870 0009-5893 73:9-10<865 2011 73 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer