caa a22 4500 445803010 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2071-x doi (NATIONALLICENCE)springer-10.1007/s10337-011-2071-x Determination of Fulvestrant in Rat Plasma by LC-MS-MS: Application to a Pharmacokinetic Study [Elektronische Daten] [Zhongyang Liu, Guangzhi Gu, Huimin Xia, Qiang Liu, Zhiqing Pang, Xinguo Jiang, Jun Chen] A reversed-phase liquid chromatography coupled to tandem mass spectrometry (LC-MS-MS) method was developed and validated for the determination of fulvestrant in rat plasma. Sample preparation involved a liquid-liquid extraction using 1.0mL of n-hexane-isopropanol (90:10, v/v) to extract the analyte from 0.1mL of rat plasma. The analytes were separated on a phenyl-based column using the mobile phase consisting of methanol/water containing 5mM ammonium acetate at the flow rate of 0.3mLmin−1. The analytes were monitored by tandem mass spectrometry under electrospray negative ionization mode. Linear calibration curves were generated over the fulvestrant concentration ranges of 0.05-10.0ngmL−1 in rat plasma. The accuracy and within- and between-day precisions were within the generally accepted criteria for bioanalytical methods (<15%). This developed and validated assay method was successfully employed to characterize the plasma concentration-time profile of fulvestrant after its intramuscular administration in rats at a dose of 10mgkg−1. Springer-Verlag, 2011 LC-MS-MS nationallicence Fulvestrant nationallicence Pharmacokinetics nationallicence Liu Zhongyang Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut Gu Guangzhi Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut Xia Huimin Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut Liu Qiang School of Pharmacy, Fujian University of Traditional Chinese Medicine, 350108, Fuzhou, Fujian, People's Republic of China aut Pang Zhiqing Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut Jiang Xinguo Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut Chen Jun Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 227-234 0009-5893 74:3-4<227 2011 74 10337 https://doi.org/10.1007/s10337-011-2071-x text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2071-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liu Zhongyang Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Gu Guangzhi Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Xia Huimin Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Liu Qiang School of Pharmacy, Fujian University of Traditional Chinese Medicine, 350108, Fuzhou, Fujian, People's Republic of China aut NATIONALLICENCE 700 1- Pang Zhiqing Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Jiang Xinguo Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 700 1- Chen Jun Department of Pharmaceutics, School of Pharmacy, Fudan University, 201203, Shanghai, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 227-234 0009-5893 74:3-4<227 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer