caa a22 4500 445803029 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2051-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2051-1 Extraction of Azole Antifungal Drugs from Milk and Biological Fluids Using a New Hollow Fiber Liquid-Phase Microextraction and Analysis by GC-FID [Elektronische Daten] [Behnam Ebrahimpour, Yadollah Yamini, Ali Esrafili] Hollow fiber liquid-phase microextraction (HF-LPME) based on two immiscible organic solvents (n-dodecane as membrane solvent and 22μL acetonitrile as acceptor phase) was evaluated for extraction and preconcentration of trace amounts of miconazole and clotrimazole as model azole antifungal drugs, prior to determination by gas chromatography (GC). The drugs were extracted from 24mL sample solution into a thin layer of organic phase (n-dodecane) immobilized in hollow fiber pores, and then back-extracted into approximately 22μL organic phase (acetonitrile) located in the lumen of the hollow fiber. After extraction, 1μL acceptor phase was injected into a GC instrument. Under optimum conditions, limits of detection (LODs) ranged from 0.3-6.0ngmL−1, linear dynamic range of 1-400ngmL−1 and relative standard deviations (RSD%) <5% were obtained. The developed method provided preconcentration factors of 639 and 496 for clotrimazole and miconazole, respectively. The applicability of the extraction method to different types of complicated matrices such as milk, plasma and urine samples was investigated. The results obtained results reveal that the proposed method is a good technique for extraction and determination of the target drugs in different matrices. Springer-Verlag, 2011 Gas chromatography nationallicence Hollow fiber liquid-phase microextraction nationallicence Two immiscible organic solvents nationallicence Antifungal drugs nationallicence Ebrahimpour Behnam Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran aut Yamini Yadollah Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran aut Esrafili Ali Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 281-289 0009-5893 74:3-4<281 2011 74 10337 https://doi.org/10.1007/s10337-011-2051-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2051-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ebrahimpour Behnam Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran aut NATIONALLICENCE 700 1- Yamini Yadollah Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran aut NATIONALLICENCE 700 1- Esrafili Ali Department of Chemistry, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 281-289 0009-5893 74:3-4<281 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer