caa a22 4500 445803037 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2059-6 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2059-6 Recovery of C-Phycocyanin in the Presence of Cells Using Expanded Bed IEC [Elektronische Daten] [Caroline Moraes, Joana da Costa Ores, Jorge Costa, Susana Kalil] C-Phycocyanin is a natural blue dye that can be extracted from Spirulina platensis. In this paper, a crude extract of C-phycocyanin in the presence of cells was purified using ion exchange chromatography in an expanded bed. The elution was studied aiming at its optimization using a central composite rotatable design (CCRD), the independent variables being the pH and elution volume, and the responses purity, concentration, yield and recovery. An evaluation of the contour curves showed that the best conditions for purification were a pH value of 6.5 and elution volume of about 150mL. A run was carried out under these conditions, combining a saline gradient (0-1M) with step elution using 0.1M NaCl. Under these conditions, it was possible to obtain C-phycocyanin with a purity increment of 4.6-fold compared with the initial extract working directly with unclarified extract. Springer-Verlag, 2011 Expanded bed nationallicence Ion exchange chromatography nationallicence Purification nationallicence C-Phycocyanin nationallicence Moraes Caroline Engenharia de Alimentos, Universidade Federal do Pampa, P.O. Box 07, 96412-420, Bagé, RS, Brazil aut da Costa Ores Joana Escola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 475, 96201-900, Rio Grande, RS, Brazil aut Costa Jorge Escola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 475, 96201-900, Rio Grande, RS, Brazil aut Kalil Susana Escola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 475, 96201-900, Rio Grande, RS, Brazil aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 307-312 0009-5893 74:3-4<307 2011 74 10337 https://doi.org/10.1007/s10337-011-2059-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2059-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moraes Caroline Engenharia de Alimentos, Universidade Federal do Pampa, P.O. Box 07, 96412-420, Bagé, RS, Brazil aut NATIONALLICENCE 700 1- da Costa Ores Joana Escola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 475, 96201-900, Rio Grande, RS, Brazil aut NATIONALLICENCE 700 1- Costa Jorge Escola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 475, 96201-900, Rio Grande, RS, Brazil aut NATIONALLICENCE 700 1- Kalil Susana Escola de Química e Alimentos, Universidade Federal do Rio Grande, P.O. Box 475, 96201-900, Rio Grande, RS, Brazil aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 307-312 0009-5893 74:3-4<307 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer