caa a22 4500 445803045 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2066-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2066-7 Quantification of Doxorubicin in Pegylated Polymer Micelles in Rat Plasma by Methanol Precipitation-Ultrasonic Emulsion Breaking Method and UPLC-MS-MS [Elektronische Daten] [Wenping Zhang, Jin Sun, Zhi Feng, Xiaonan Su, Meng Zhu, Xiaofan Sui, Yan Liu, Mengying Tao, Yunqiang Sun, Guoliang Chen, Zhonggui He] This study established a new methanol precipitation-ultrasonic emulsion breaking method for extraction of doxorubicin from polymeric micelles and developed a UPLC-MS/MS method for the determination of doxorubicin in rat plasma. The emulsion breaking efficiency of methanol is up to 99.2%. The plasma samples were analyzed by UPLC/MS/MS using positive electrospray ionization mode in the multiple-reaction monitoring (MRM) mode. The calibration curves were linear over the range 5-1,000ngmL−1 with the lower limit of quantification of 5ngmL−1. The intra- and inter-day precisions of QC samples were all <10.0%. The chromatographic separation was 2.5min. The developed method was successfully applied to a pharmacokinetic study of doxorubicin in rats following intravenous administration. Springer-Verlag, 2011 UPLC/MS/MS nationallicence Doxorubicin nationallicence mPEG-glyceryl distearate micelles nationallicence Methanol precipitation-ultrasonic emulsion breaking method nationallicence Pharmacokinetic study nationallicence Zhang Wenping Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Sun Jin Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Feng Zhi Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Su Xiaonan Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Zhu Meng Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Sui Xiaofan Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Liu Yan Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Tao Mengying Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Sun Yunqiang Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Chen Guoliang Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut He Zhonggui Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 333-340 0009-5893 74:3-4<333 2011 74 10337 https://doi.org/10.1007/s10337-011-2066-7 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2066-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Wenping Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Sun Jin Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Feng Zhi Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Su Xiaonan Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Zhu Meng Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Sui Xiaofan Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Liu Yan Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Tao Mengying Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Sun Yunqiang Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Chen Guoliang Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 700 1- He Zhonggui Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, 110016, Shenyang, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 333-340 0009-5893 74:3-4<333 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer