caa a22 4500 445803053 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2062-y doi (NATIONALLICENCE)springer-10.1007/s10337-011-2062-y Extrathermodynamic Study of Retention Equilibrium in RP-LC Using a C18-Silica Monolithic Stationary Phase [Elektronische Daten] [Kanji Miyabe, Akane Matsushima] This study is concerned with the explanation of some thermodynamic properties of the retention equilibrium on a C18-silica monolithic column. Pulse response experiments were carried out in a reversed-phase liquid chromatography system using a methanol/water mixture (70/30, v/v) and n-alkylbenzene homologs as the mobile phase and sample compounds, respectively, in the temperature range between 278 and 318K. The retention equilibrium constant (K a) was calculated from the first absolute moment of elution peaks. The dependence of K a on the column temperature was analyzed using the modified van't Hoff plot proposed by Krug et al. to derive the changes of the Gibbs free energy, the enthalpy and the entropy concerning the retention behavior. First, the presence of a real enthalpy-entropy compensation (EEC) for the retention equilibrium was demonstrated. Then, a thermodynamic model based on the real EEC was developed to explain the temperature dependence of the linear free energy relationship (LFER) of the retention equilibrium. The model indicates how the slope and intercept of the LFER are correlated with the compensation temperatures and several molecular thermodynamic parameters. The model was effective for explaining the thermodynamic properties of the retention equilibrium of the C18-silica monolithic stationary phase. Springer-Verlag, 2011 Reversed-phase liquid chromatography nationallicence Monolithic stationary phase nationallicence Retention equilibrium nationallicence Thermodynamics nationallicence Enthalpy-entropy compensation nationallicence Linear free energy relationship nationallicence Miyabe Kanji Graduate School of Science and Engineering for Research, University of Toyama, 3190, Gofuku, 930-8555, Toyama, Japan aut Matsushima Akane Faculty of Engineering, University of Toyama, 3190, Gofuku, 930-8555, Toyama, Japan aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 179-187 0009-5893 74:3-4<179 2011 74 10337 https://doi.org/10.1007/s10337-011-2062-y text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2062-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miyabe Kanji Graduate School of Science and Engineering for Research, University of Toyama, 3190, Gofuku, 930-8555, Toyama, Japan aut NATIONALLICENCE 700 1- Matsushima Akane Faculty of Engineering, University of Toyama, 3190, Gofuku, 930-8555, Toyama, Japan aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 179-187 0009-5893 74:3-4<179 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer