caa a22 4500 445803088 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2061-z doi (NATIONALLICENCE)springer-10.1007/s10337-011-2061-z Determination of Albuterol Enantiomers in Animal Tissue Matrices by LC-MS/MS: Application in Therapeutic Myoanabolic Studies [Elektronische Daten] [Kwang Yee, Edwin Lowe, Glenn Jacobson] Albuterol (salbutamol) is a widely used medication in respiratory disease including asthma and chronic obstructive airways disease; however, like other beta2-agonists, it also exerts some extrapulmonary effects on muscle and fat. Surprisingly, there have been relatively few reports of albuterol tissue distribution, and the distribution of individual albuterol enantiomers into tissue has not been reported. The method presented here explores the use of an HPLC tandem mass spectrometry (LC-MS/MS) system (LTQ Orbitrap hybrid mass spectrometer) with deuterated standard and solid-phase extraction to determine low levels of albuterol enantiomers in tissue. The lower limit of quantification (LLOQ) was 0.156ngg−1, with a precision RSD <15% for both enantiomers. The assay was linear over the calibration range of LLOQ-10.0ngg−1 in muscle tissue (r 2>0.98). The assay was successfully applied to pharmacokinetic studies in rats and mice. By utilizing a deuterated internal standard and LC-MS/MS detection, this assay can be used to measure albuterol enantiomers in muscle tissue. This assay has also identified that albuterol uptake appears to be stereoselective, and enantioselective assays are clearly warranted for myoanabolic studies involving administration of racemic-albuterol. Springer-Verlag, 2011 Salbutamol nationallicence Chiral nationallicence Animal tissue nationallicence Liquid chromatography-tandem mass spectrometry nationallicence Yee Kwang School of Pharmacy, University of Tasmania, Private Bag 26, 7001, Hobart, TAS, Australia aut Lowe Edwin Central Science Laboratory, University of Tasmania, Private Bag 74, 7001, Hobart, TAS, Australia aut Jacobson Glenn School of Pharmacy, University of Tasmania, Private Bag 26, 7001, Hobart, TAS, Australia aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 355-358 0009-5893 74:3-4<355 2011 74 10337 https://doi.org/10.1007/s10337-011-2061-z text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2061-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yee Kwang School of Pharmacy, University of Tasmania, Private Bag 26, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Lowe Edwin Central Science Laboratory, University of Tasmania, Private Bag 74, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 700 1- Jacobson Glenn School of Pharmacy, University of Tasmania, Private Bag 26, 7001, Hobart, TAS, Australia aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 355-358 0009-5893 74:3-4<355 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer