caa a22 4500 445803118 CHVBK 20180317145147.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2056-9 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2056-9 Purification of IgG from Sera of Rabbit and Guinea Pig by Flow-Through Mode Ion-Exchange Chromatography using DEAE Sepharose Fast Flow Column [Elektronische Daten] [Ying Wang, Peiyin Zhang, Shujun Liu, Yongsheng Zhang, Tiesuo Zhao, Wenhui Huang, Chunyan He, Yongli Yu, Liying Wang, Min Wan] A one-step purification process using flow-through mode ion-exchange chromatography (Ft-IEC) was evaluated for the purification of Immunoglobulin G (IgG) from rabbit and guinea pig serum. A simple protein precipitation with saturated ammonium sulfate was used to pretreated plasma samples. Chromatographic separation was carried out on DEAE Sepharose Fast Flow (F.F.) column at a flow rate of 1mLmin−1. Ft-IEC using Tris-HCl buffer at pH 7.0 allowed the recovery of 22% of the loaded IgG with purity of 95% existed in flowthrough and washing effluents but not in elution effluents. To be compared, bind-elute mode IEC using Tris-HCl buffer at pH 8.5 based on the routine protocol showed that the recovery of 15% of the loaded IgG with purity of 80% existed in elution effluents. These results indicate that the Ft-IEC is an effective method for purifying IgG from sera of rabbit and guinea pig and may also be suitable for other animal sera by adjusting pH at equilibrium buffer for chromatography column. Springer-Verlag, 2011 Flow-through mode ion-exchange chromatography nationallicence Immunoglobulin G (IgG) nationallicence Purification nationallicence pH value nationallicence Wang Ying Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Zhang Peiyin Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Liu Shujun Department of Nephropathy, The Second Hospital, Jilin University, 130021, Changchun, China aut Zhang Yongsheng Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Zhao Tiesuo Department of Immunology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Huang Wenhui Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut He Chunyan Department of Immunology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Yu Yongli Department of Immunology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Wang Liying Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Wan Min Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 209-214 0009-5893 74:3-4<209 2011 74 10337 https://doi.org/10.1007/s10337-011-2056-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2056-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Ying Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Zhang Peiyin Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Liu Shujun Department of Nephropathy, The Second Hospital, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Zhang Yongsheng Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Zhao Tiesuo Department of Immunology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Huang Wenhui Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- He Chunyan Department of Immunology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Yu Yongli Department of Immunology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Wang Liying Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 700 1- Wan Min Department of Molecular Biology, Norman Bethune College of Medicine, Jilin University, 130021, Changchun, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 209-214 0009-5893 74:3-4<209 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer