caa a22 4500 445803169 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2064-9 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2064-9 Multiobjective Optimization Approach in Evaluation of Chromatographic Behaviour of Zolpidem Tartrate and Its Degradation Products [Elektronische Daten] [Marija Malesevic, Ljiljana Zivanovic, Ana Protic, Zarko Jovic] Multiresponse optimization methodology in combination with experimental design was employed as a powerful technique for simultaneous optimization of input variables significant for evaluation of chromatographic behaviour of zolpidem tartrate, zolpacid, oxozolpidem, zolpyridine and zolpaldehyde towards various responses. In the first stage of the investigation fractional factorial design was used to decrease the number of variables that should be studied in detail. Among examined variables, pH of the mobile phase, percentage of organic modifier and buffer concentration showed to be statistically important and were consequently optimized with central composite design and Derringer's desirability function. Four responses were considered, the retention factors of zolpacid and zolpaldehyde (the first and last peak) and the resolutions between critical peaks. Optimal conditions included Luna C18(2) analytical column (250mmx4.6mm, 5μm particle size), mobile phase consisted of methanol-10mM ammonium acetate (68.4:31.6, v/v, pH 5.4) and column temperature of 35°C. The flow rate of the mobile phase was 1mLmin−1 and the detection was performed at 254nm. At the end, the method was successfully validated in accordance with ICH guideline and subsequently applied to the analysis of commercially available zolpidem tartrate tablets. Springer-Verlag, 2011 Column liquid chromatography nationallicence Fractional factorial design nationallicence Central composite design nationallicence Derringer's desirability function nationallicence Zolpidem tartrate nationallicence Malesevic Marija Medicines and Medical Devices Agency of Serbia, Vojvode Stepe 458, 11000, Belgrade, Serbia aut Zivanovic Ljiljana Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000, Belgrade, Serbia aut Protic Ana Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000, Belgrade, Serbia aut Jovic Zarko Medicines and Medical Devices Agency of Serbia, Vojvode Stepe 458, 11000, Belgrade, Serbia aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 197-208 0009-5893 74:3-4<197 2011 74 10337 https://doi.org/10.1007/s10337-011-2064-9 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2064-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Malesevic Marija Medicines and Medical Devices Agency of Serbia, Vojvode Stepe 458, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Zivanovic Ljiljana Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Protic Ana Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, P.O. Box 146, 11000, Belgrade, Serbia aut NATIONALLICENCE 700 1- Jovic Zarko Medicines and Medical Devices Agency of Serbia, Vojvode Stepe 458, 11000, Belgrade, Serbia aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 197-208 0009-5893 74:3-4<197 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer