caa a22 4500 445803177 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2073-8 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2073-8 Brain Distribution Study of Imperatorin in Rats after Oral Administration Assessed by HPLC [Elektronische Daten] [Xiaokai Zhang, Yanhua Xie, Wei Cao, Qian Yang, Shan Miao, Siwang Wang] Imperatorin (IMP) is a biologically active ingredient isolated from a traditional Chinese medicine (TCM), Angelica dahurica. To obtain the brain distribution data of IMP in rats, the concentrations of IMP in cortex, cerebellum, diencephalon, brain stem, striatum and hippocampus were measured by a simple and sensitive HPLC-UV method. The analytes were prepared by a liquid-liquid extraction method and the separation of IMP was performed on a Hypersil BDS C18 column (250mm×4.6mm i.d., 5μm) using acetonitrile-water (60:40, v/v) as mobile phase which was delivered at 1.0mLmin−1. Ultraviolet detection was performed at 300nm. Using a weighted (1/c2) least square method, linear calibration curves for the six regions were obtained (r≥0.9990) with a lower limit of quantification (LLOQ) of 0.075μgg−1 for cortex, cerebellum, diencephalon and brain stem or 0.15μgg−1 for striatum and hippocampus, and the recovery was greater than 90% for each tissue sample. The within- and between-day precisions (expressed as the relative standard deviation, RSD) were less than 10%. The validated method has been successfully applied to the brain distribution study in rats. The results showed that IMP could pass through the blood-brain barrier easily. And the higher concentration in striatum and hippocampus compared with the others might indicate that they were the target regions of IMP in rat brain. Springer-Verlag, 2011 HPLC-UV nationallicence Brain distribution nationallicence Imperatorin nationallicence Angelica dahurica nationallicence Zhang Xiaokai Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut Xie Yanhua Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut Cao Wei Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut Yang Qian Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut Miao Shan Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut Wang Siwang Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 259-265 0009-5893 74:3-4<259 2011 74 10337 https://doi.org/10.1007/s10337-011-2073-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2073-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Xiaokai Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Xie Yanhua Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Cao Wei Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Yang Qian Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Miao Shan Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 700 1- Wang Siwang Institute of Materia Medica, School of Pharmacy, Fourth Military Medical University, 169 West Changle Road, 710032, Xi'an, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 259-265 0009-5893 74:3-4<259 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer