caa a22 4500 445803193 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2057-8 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2057-8 LC Determination of Icariside II in Rat Plasma and Tissues: Application to a Tissue Distribution Study [Elektronische Daten] [Chang-Guang Wang, Jia-Bei Sun, Jian-Cheng Wang, Qiang Zhang, Ying Zheng] A sensitive and reliable reversed-phase liquid chromatography (RP-LC) with ultraviolet (UV) detection has been developed and validated for the quantification of Icariside II in rat plasma and tissues using Fermononetin as the internal standard. Protein precipitation and liquid-liquid extraction were utilized for plasma and tissue sample preparation, respectively. The analysis was successfully carried out on an Agilent SB-C18 column (5μm, 4.6×250mm) with the implementation of the following conditions: a mobile phase of phosphoric acid solution (0.1%, v/w)-Acetonitrile (55:45, v/v), a flow rate of 1mLmin−1, a column temperature of 25°C and a detection wavelength of 270nm. Good linear relationships of calibration curves were obtained (r 2>0.9906) over the investigated concentration range with plasma and tissue samples. The lower limit of quantification (LLOQ) and the limit of detection (LOD) were 0.1 and 0.02μgg−1, respectively (for plasma sample, they were 0.05 and 0.1μgmL−1, respectively). The developed method which was embodied with good precision, accuracy, recovery and stability was corroborated to satisfy the requirements for biomedical sample analysis. This method has been successfully applied to tissue distribution study of Icariside II in rats after a single intravenous dose at 12.5mgkg−1. Results suggested that Icariside II was distributed to rat tissues rapidly with greater initial concentrations in kidney, lung and liver. Moderate initial distributions were obtained in rat muscle, heart, bone, spleen and plasma. Low amount of Icariside II was detected in testes, and no Icariside II could be detected in the brain. Springer-Verlag, 2011 Column liquid chromatography-ultraviolet detection nationallicence Icariside II nationallicence Flavonoids nationallicence Tissue distribution nationallicence Wang Chang-Guang Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China aut Sun Jia-Bei Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China aut Wang Jian-Cheng Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut Zhang Qiang Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut Zheng Ying Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 251-258 0009-5893 74:3-4<251 2011 74 10337 https://doi.org/10.1007/s10337-011-2057-8 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2057-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Chang-Guang Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China aut NATIONALLICENCE 700 1- Sun Jia-Bei Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China aut NATIONALLICENCE 700 1- Wang Jian-Cheng Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Zhang Qiang Department of Pharmaceutics, School of Pharmaceutical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Zheng Ying Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 251-258 0009-5893 74:3-4<251 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer