caa a22 4500 445803207 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2033-3 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2033-3 UPLC-MS-MS Method for the Determination of N -(2,6-Dimethoxypyridine-3-yl)-9-methylcarbazole-3-sulfonamide in Rat Plasma and Its Application to a Pharmacokinetic Study [Elektronische Daten] [Xin Li, Yinghua Sun, Xiaohong Liu, Yanhua Liu, Jin Sun, Zhonggui He] The purpose of the study is first to develop a sensitive and rapid ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method for the determination of a new synthesized tubulin ligand, N-(2,6-dimethoxypyridine-3-yl)-9-methylcarbazole-3-sulfonamide (IG-105), in rat plasma. The analyte and internal standard (carbamazepine) were extracted by liquid/liquid extraction with petroleum ether/diethyl ether (2:1, v/v). The chromatographic separation was performed on an Acquity UPLC BEH C18 column with a mobile phase gradient consisting of methanol and water. The mass spectrometric detection was performed by triple-quadrupole mass spectrometry with multiple reaction monitoring (MRM) via an ESI source operating in positive ionization mode. The mass transitions m/z 398→153 and m/z 237→194 were used to assay the analyte and IS, respectively. The method demonstrated good linearity over a concentration range of 0.67-333.33ngmL−1, and the lower limit of quantitation (LLOQ) of IG-105 was 0.67ngmL−1. The intra- and inter-day precision (relative standard deviation) values were <6%, and the accuracy (relative error) was <5% at three quality control levels. The extraction recovery of IG-105 and IS was 84.45 and 88.5%, respectively. Finally, the validated method was successfully applied to a pharmacokinetic study of IG-105 in rat plasma. Springer-Verlag, 2011 UPLC-MS-MS nationallicence IG-105 nationallicence Rat plasma nationallicence Pharmacokinetic study nationallicence Li Xin Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut Sun Yinghua Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut Liu Xiaohong Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut Liu Yanhua Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut Sun Jin Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut He Zhonggui Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 327-331 0009-5893 74:3-4<327 2011 74 10337 https://doi.org/10.1007/s10337-011-2033-3 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2033-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Xin Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Sun Yinghua Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Liu Xiaohong Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Liu Yanhua Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut NATIONALLICENCE 700 1- Sun Jin Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut NATIONALLICENCE 700 1- He Zhonggui Department of Biopharmaceutics, School of Pharmacy, Shenyang Pharmaceutical University, No. 103, Wenhua Road, Mailbox 59, 110016, Shenyang, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 327-331 0009-5893 74:3-4<327 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer