caa a22 4500 445803223 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2060-0 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2060-0 Determination of Spectinomycin in Human Urine Using CE Coupled with Electrogenerated Chemiluminescence [Elektronische Daten] [Wei Wei, Min Wei, Zeyu Cai, Songqin Liu] A method of capillary electrophoresis with electrogenerated chemiluminescence was developed for the detection of spectinomycin. The linear ranges were from 0.01 to 1.0mgmL−1 for spectinomycin. The limit of detection for spectinomycin with a signal-to-noise ratio of 3:1 was found to be 4.0µgmL−1. For practical application perchloric acid was used to eliminate the protein contained in human urine, which is very harmful to electrophoresis separation. The recoveries of spectinomycin at different levels in human urine were between 91.1 and 106.5%. The RSD was between 2.6 and 4.8% (n=5-6). It was valuable in clinical and biochemical laboratories for monitoring the drug for various purposes. Springer-Verlag, 2011 Capillary electrophoresis nationallicence Electrogenerated chemiluminescence nationallicence Spectinomycin nationallicence Wei Wei College of Chemistry and Chemical Engineering, Southeast University, 210009, Nanjing, People's Republic of China aut Wei Min College of Food Science and Technology, Henan University of Technology, 450052, Zhengzhou, People's Republic of China aut Cai Zeyu Gulou Center for Disease Control and Prevention, 210009, Nanjing, People's Republic of China aut Liu Songqin College of Chemistry and Chemical Engineering, Southeast University, 210009, Nanjing, People's Republic of China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 349-353 0009-5893 74:3-4<349 2011 74 10337 https://doi.org/10.1007/s10337-011-2060-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2060-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Wei College of Chemistry and Chemical Engineering, Southeast University, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Wei Min College of Food Science and Technology, Henan University of Technology, 450052, Zhengzhou, People's Republic of China aut NATIONALLICENCE 700 1- Cai Zeyu Gulou Center for Disease Control and Prevention, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Songqin College of Chemistry and Chemical Engineering, Southeast University, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 349-353 0009-5893 74:3-4<349 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer