caa a22 4500 445803231 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2058-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2058-7 Study on the Effect of Moderate Exercise on Lactic Acid Content in Breast Milk by Indirect CE with Amperometric Detection [Elektronische Daten] [Meijiang Zhang, Yiping Wang, Junbo Zhang, Qian Cui, Jiannong Ye, Qingcui Chu] An indirect high-performance capillary electrophoresis with amperometric detection (CE-AD) method has been developed for determination of lactic acid (LA) in body fluids of lactating postpartum women. Several important factors such as the running buffer additive and concentration, the working electrode potential, the pH value and concentration of the running buffer, the separation voltage and injection time were investigated. Under the optimum conditions, LA could be well separated with co-existing interferences including uric acid (UA) in real samples in a 90-cm-length capillary at separation voltage of 12kV in 4.0×10−6gmL−1 3,4-dihydroxybenzylamine (DHBA)/40mmolL−1 H3BO3-Na2B4O7 buffer (pH 7.8). The linearity between peak current and concentration of LA was over three orders of magnitude with detection limit of 5.00×10−7gmL−1 (S/N=3). This proposed method has been successfully used to study the effects of moderate exercise on LA content in breast milk and urine samples of lactating postpartum women, and assay results showed that LA content in breast milk can return to normal level through 60min rest without decreasing acceptance by breast-feeding infants, although the LA level did increase by 4-6 times in both breast milk and urine samples at 10min after moderate exercise. Springer-Verlag, 2011 Capillary electrophoresis nationallicence Indirect amperometric detection nationallicence Lactic acid nationallicence Breast milk nationallicence Urine nationallicence Moderate exercise nationallicence Zhang Meijiang School of Sports and Health Care, East China Normal University, 200062, Shanghai, China aut Wang Yiping Department of Chemistry, East China Normal University, 200062, Shanghai, China aut Zhang Junbo Department of Chemistry, East China Normal University, 200062, Shanghai, China aut Cui Qian Department of Chemistry, East China Normal University, 200062, Shanghai, China aut Ye Jiannong Department of Chemistry, East China Normal University, 200062, Shanghai, China aut Chu Qingcui Department of Chemistry, East China Normal University, 200062, Shanghai, China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 275-280 0009-5893 74:3-4<275 2011 74 10337 https://doi.org/10.1007/s10337-011-2058-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2058-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Meijiang School of Sports and Health Care, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Wang Yiping Department of Chemistry, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Zhang Junbo Department of Chemistry, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Cui Qian Department of Chemistry, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Ye Jiannong Department of Chemistry, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 700 1- Chu Qingcui Department of Chemistry, East China Normal University, 200062, Shanghai, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 275-280 0009-5893 74:3-4<275 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer