caa a22 4500 44580324X CHVBK 20180317145148.0 cr unu---uuuuu 170323e20110801xx s 000 0 eng 10.1007/s10337-011-2053-z doi (NATIONALLICENCE)springer-10.1007/s10337-011-2053-z Simultaneous Determination of Escin Ia and Its Isomer Isoescin Ia by LC-MS-MS: Application to a Pharmacokinetic Study of Escin Ia in Rats [Elektronische Daten] [Mengliang Zhang, Xiujun Wu, Xiangyong Cui, Feng Gao, Chao Zhang, Yan Yang, Jingkai Gu] A rapid and sensitive LC-MS-MS method for the simultaneous determination of escin Ia and isoescin Ia in rat plasma, urine, feces and bile samples was developed and validated. Analytes and telmisartan [internal standard (IS)] were extracted by solid-phase extraction on C18 cartridges. Components in the extract were separated on an HC-C18 column (5μm, 150×4.6mm i.d.) using 10mM ammonium acetate-methanol-acetonitrile (40:30:30, v/v/v) as the mobile phase. The method demonstrated good linearity from 5ngmL−1 (LLOQ) to 1,500ngmL−1 for both escin Ia and isoescin Ia. Intra- and inter-day precision measured as RSD was within ±15%. Recoveries and matrix effects of both escin Ia and isoescin Ia were satisfactory in all four matrices examined. The method was successfully applied to a pharmacokinetic study in Wistar rats after a single intravenous administration of escin Ia at the dose of 1.0mgkg−1. Springer-Verlag, 2011 LC-MS-MS nationallicence Escin Ia nationallicence Isoescin Ia nationallicence Pharmacokinetics nationallicence Zhang Mengliang Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut Wu Xiujun Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut Cui Xiangyong Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut Gao Feng Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut Zhang Chao Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut Yang Yan College of Life Science, Jilin University, 130023, Changchun, People's Republic of China aut Gu Jingkai Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut Chromatographia Springer-Verlag 74/3-4(2011-08-01), 243-250 0009-5893 74:3-4<243 2011 74 10337 https://doi.org/10.1007/s10337-011-2053-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2053-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhang Mengliang Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Wu Xiujun Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Cui Xiangyong Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Gao Feng Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Chao Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Yang Yan College of Life Science, Jilin University, 130023, Changchun, People's Republic of China aut NATIONALLICENCE 700 1- Gu Jingkai Research Center for Drug Metabolism, Jilin University, 130023, Changchun, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/3-4(2011-08-01), 243-250 0009-5893 74:3-4<243 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer