caa a22 4500 445803266 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2110-7 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2110-7 Determination of Rizatriptan in Human Plasma by Liquid Chromatography Stable Isotope Dilution Electrospray MS-MS for Application in Bioequivalence Study [Elektronische Daten] [Ramakotaiah Mogili, Kanchanamala Kanala, Balasekhara Challa, Babu Chandu, Chandrasekhar Bannoth] A simple, sensitive, selective, rapid, rugged, reproducible and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for quantitative estimation of rizatriptan (RZ) in human plasma using rizatriptan-d 6 (RZD6) as internal standard (IS). Chromatographic separation was performed on Ascentis Express RP Amide C18, 50×4.6mm, 2.7μm column with isocratic mobile phase composed of 10mM ammonium formate:acetonitrile (20:80 v/v) at flow rate of 0.5mLmin−1. RZ and RZD6 were detected with proton adducts at m/z (amu) 270.2→201.2 and 276.1→207.1, respectively, in multiple reaction monitoring (MRM) positive mode. Liquid-liquid extraction was used and validated over a linear concentration range of 0.1-100.0ngmL−1 with correlation coefficient r 2≥0.9981. The limit of quantification (LOQ) and limit of detection (LOD) were found to be 0.1ngmL−1 and 12.5fg, respectively. Intra- and inter-day precision were within 1.7-3.1% and 2.8-3.7%, and accuracy within 96.0-101.7% and 99.7-101.4% for RZ. Drug was found to be stable throughout three freeze-thaw cycles. The method was successfully employed for analysis of plasma samples following oral administration of RZ (10mg) in 25healthy Indian male human volunteers under fasting conditions. Springer-Verlag, 2011 Column liquid chromatography nationallicence Mass spectrometry nationallicence Rizatriptan nationallicence Human plasma nationallicence Bioequivalence nationallicence Mogili Ramakotaiah Jawaharlal Nehru Technological University, 515002, Anantapur, AP, India aut Kanala Kanchanamala Jawaharlal Nehru Technological University, 515002, Anantapur, AP, India aut Challa Balasekhara Nirmala College of Pharmacy, 516002, Kadapa, AP, India aut Chandu Babu Donbosco P.G. College of Pharmacy, Pulladigunta, Guntur, AP, India aut Bannoth Chandrasekhar Jawaharlal Nehru Technological University, 515002, Anantapur, AP, India aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 585-592 0009-5893 74:7-8<585 2011 74 10337 https://doi.org/10.1007/s10337-011-2110-7 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2110-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mogili Ramakotaiah Jawaharlal Nehru Technological University, 515002, Anantapur, AP, India aut NATIONALLICENCE 700 1- Kanala Kanchanamala Jawaharlal Nehru Technological University, 515002, Anantapur, AP, India aut NATIONALLICENCE 700 1- Challa Balasekhara Nirmala College of Pharmacy, 516002, Kadapa, AP, India aut NATIONALLICENCE 700 1- Chandu Babu Donbosco P.G. College of Pharmacy, Pulladigunta, Guntur, AP, India aut NATIONALLICENCE 700 1- Bannoth Chandrasekhar Jawaharlal Nehru Technological University, 515002, Anantapur, AP, India aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 585-592 0009-5893 74:7-8<585 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer