caa a22 4500 445803274 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2120-5 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2120-5 Preparation and Evaluation of Poly- l -Lysine Stationary Phase for Hydrophilic Interaction/Reversed-Phase Mixed-Mode Chromatography [Elektronische Daten] [Yuanyuan Li, Zhigang Xu, Yaoyao Feng, Xiaoyan Liu, Tong Chen, Haixia Zhang] In this work, a poly-l-lysine-grafted stationary phase was synthesized by polymerization of N-carboxyanhydride of l-lysine initiated by 3-aminopropylated silica. The resulting material was characterized by FT-IR spectra, elemental analysis and thermogravimetric analysis, which clearly indicated that the new phase had been prepared successfully. The retention of polar solutes depending on acetonitrile content in mobile phase exhibited "U-shaped” curves, which was an indication of hydrophilic interaction liquid chromatography (HILIC)/reversed-phase liquid chromatography (RPLC) mixed-mode retention behavior. The retention mechanisms in HILIC and RPLC modes also were investigated. Phenol compounds, aniline compounds and hydrophilic compounds were separated in RPLC or HILIC mode on the new stationary phase, respectively. This result shows that the new phase could be used for both RPLC and HILIC applications, providing greater flexibility for real sample analysis. Springer-Verlag, 2011 Hydrophilic interaction liquid chromatography nationallicence Mixed-mode stationary phase nationallicence Poly- l -lysine nationallicence Polar solutes nationallicence Li Yuanyuan Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut Xu Zhigang Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut Feng Yaoyao Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut Liu Xiaoyan Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut Chen Tong Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut Zhang Haixia Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 523-530 0009-5893 74:7-8<523 2011 74 10337 https://doi.org/10.1007/s10337-011-2120-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2120-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Li Yuanyuan Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Xu Zhigang Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Feng Yaoyao Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Liu Xiaoyan Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Chen Tong Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 700 1- Zhang Haixia Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou University, 730000, Lanzhou, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 523-530 0009-5893 74:7-8<523 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer