caa a22 4500 445803290 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2095-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2095-2 Simultaneous Determination of Buprenorphine, Norbuprenorphine and Naloxone in Human Plasma by LC-MS-MS [Elektronische Daten] [Ting-Yi Chiang, Li-Heng Pao, Cheng-Huei Hsiong, Pei-Wei Huang, Kai-Wei Lin, Oliver Hu] A novel sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS-MS) method simultaneously determined buprenorphine (BUP) and its active metabolite, norbuprenorphine (NBUP), and a coformulant, naloxone was developed, validated and applied successfully in humans. Buprenorphine-d 4 and norbuprenorphine-d 3 were used as the internal standard. The analysis was performed on a silica column, and the mobile phase was isocratic and composed of acetonitrile:2mM ammonium formate in H2O (82:18, v/v). Mass spectrometry employed multiple reaction monitoring modes with transitions of m/z 468.1-55.2 for BUP, 414.2-101.2 for NBUP, 328.3-310.3 for naloxone, 472.1-59.2 for buprenorphine-d 4 and 417.2-101.2 for norbuprenorphine-d 3. Lower limit of quantification (LLOQ) of the analytical method was 0.05ngmL−1 for BUP, 0.1ngmL−1 for NBUP and 0.025ngmL−1 for naloxone, respectively. The standard calibration curves of BUP, NBUP and naloxone were linear over the concentration range of 0.05-20ngmL−1, 0.1-20ngmL−1 and 0.025-20ngmL−1, respectively. The precisions (RSD) and accuracies (RE) of LLOQ and other QC samples were in acceptable range, with RSD<20% and RE±20% for LLOQ and RSD<15% and RE within ±15% for QC samples. The method was accurate, precise and specific, and was applied to the pharmacokinetic study of buprenorphine in healthy volunteers. Springer-Verlag, 2011 LC-MS-MS nationallicence Pharmacokinetics nationallicence Buprenorphine nationallicence Norbuprenorphine nationallicence Naloxone nationallicence Chiang Ting-Yi Graduate Institute of Medical Sciences, National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut Pao Li-Heng National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut Hsiong Cheng-Huei National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut Huang Pei-Wei National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut Lin Kai-Wei Graduate Institute of Life Sciences, National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut Hu Oliver National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 575-583 0009-5893 74:7-8<575 2011 74 10337 https://doi.org/10.1007/s10337-011-2095-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2095-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chiang Ting-Yi Graduate Institute of Medical Sciences, National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Pao Li-Heng National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Hsiong Cheng-Huei National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Huang Pei-Wei National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Lin Kai-Wei Graduate Institute of Life Sciences, National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 700 1- Hu Oliver National Defense Medical Center, No.161, Section 6, Min-Chuan East Road, 114, Taipei, Taiwan, Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 575-583 0009-5893 74:7-8<575 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer