caa a22 4500 445803363 CHVBK 20180317145148.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2116-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2116-1 Simultaneous Estimation of Ceftazidime and Ceftizoxime in Pharmaceutical Formulations by HPLC Method [Elektronische Daten] [Senem Sanli, Nurullah Sanli, Mehmet Gumustas, Sibel Ozkan, Nurgul Karadas, Hassan Aboul-Enein] The aim of the present study was to develop a fast, sensitive and reliable method for rapid screening of cephalosporin injectable dosage forms namely ceftazidime and ceftizoxime to the detection of counterfeit and substandard drugs that might be illegally commercialized. Ceftazidime, ceftizoxime and cefixime (IS) were separated in a X-Terra RP-18 column (250×4.60mm ID×5μ) and DAD detector set at 290 and 260nm. The mobile phase consisted of a mixture of methanol:water 20:80 (v/v) at a flow rate of 1.0mLmin−1. Additionally, in order to find the optimum pH value of separation the pK a values of studied compounds were determined by using two different methodologies. Aqueous pK a values of studied compounds have been determined by UV-spectrophotometry and liquid chromatography were used for the determination and direct characterization of the dissociation constants by using the dependence of the capacity factor on the pH of the mobile phase in 20% (v/v) methanol-water binary mixture in which separation was performed. The pH of the mobile phase was adjusted with 25mM H3PO4 to 3.2. The method was shown to be linear, sensible, accurate, and reproducible over the range of analysis and it can be used to pharmaceutical formulations containing a single active ingredient within a short analysis time. Springer-Verlag, 2011 Column liquid chromatography nationallicence UV-spectrophotometry nationallicence Ceftazidime nationallicence Ceftizoxime nationallicence p K a values nationallicence Sanli Senem Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut Sanli Nurullah Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut Gumustas Mehmet Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut Ozkan Sibel Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Tandogan, Ankara, Turkey aut Karadas Nurgul Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut Aboul-Enein Hassan Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, 12311, Cairo, Egypt aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 549-558 0009-5893 74:7-8<549 2011 74 10337 https://doi.org/10.1007/s10337-011-2116-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2116-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sanli Senem Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut NATIONALLICENCE 700 1- Sanli Nurullah Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut NATIONALLICENCE 700 1- Gumustas Mehmet Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut NATIONALLICENCE 700 1- Ozkan Sibel Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Tandogan, Ankara, Turkey aut NATIONALLICENCE 700 1- Karadas Nurgul Department of Chemistry, Faculty of Science and Arts, Hitit University, Çorum, Turkey aut NATIONALLICENCE 700 1- Aboul-Enein Hassan Pharmaceutical and Medicinal Chemistry Department, National Research Centre, Dokki, 12311, Cairo, Egypt aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 549-558 0009-5893 74:7-8<549 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer