caa a22 4500 445803371 CHVBK 20180317145149.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2118-z doi (NATIONALLICENCE)springer-10.1007/s10337-011-2118-z Simultaneous Determination of Hydrocodone, and Its Two Metabolites in Human Plasma by HPLC-MS-MS [Elektronische Daten] [Guang-Tao Hao, Yan Chen, Rui-Hua Dong, Heng-Yan Qu, Yu-Guang Liang, Yuan-Yuan Li, Zhuan-Jie Zheng, Hong-Zhi Gao, Ze-Yuan Liu] A rapid, sensitive and specific assay method has been developed to simultaneously determine human plasma concentrations of hydrocodone and its metabolites, norhydrocodone, hydromorphone, using high-performance liquid chromatography with an electrospray ionization (ESI) tandem mass spectrometry (HPLC-MS-MS). Hydrocodone, its metabolites, and internal standard, hydrocodone-d 3, norhydrocodone-d 3, hydromorphone-d 3, were separated from human plasma using solid-phase extraction (Empore MPC-SD Solid Phase Extraction Disk). The eluate was dried, reconstituted and injected into the LC-MS-MS system. Chromatographic separation was performed on a Kromasil 100-5SIL-Dimensions C18 column (100×2.1mm, 5.0μm, Thermo Hypersil-Keystone, USA) using a gradient mobile phase with 20mmolL−1 ammonium formate in water with 0.2% formic acid and 0.1% formic acid in acetonitrile. Detection and quantitation were performed by MS/MS using electrospray ionization and multiple reactions monitoring in the positive ion mode. The calibration curves were linear over the concentration ranges 0.05-50ngmL−1 for hydrocodone (r 2=0.9991) and norhydrocodone (r 2=0.9990), and 0.01-10ngmL−1 for hydromorphone (r 2=0.9990). The limit of quantification was 0.05ngmL−1 for hydrocodone and norhydrocodone, and 0.01ngmL−1 for hydromorphone. The extraction recovery was above 64.36, 68.51 and 71.78% for hydrocodone, norhydrocodone and hydromorphone. The accuracy was higher than 99.06, 97.70 and 100.07% for hydrocodone, norhydrocodone and hydromorphone. The intra- and inter-day precisions were <5.80, 5.90 and 3.02% for hydrocodone, norhydrocodone and hydromorphone. The method was accurate, sensitive and simple and was successfully applied to a pharmacokinetic study after a single oral administration of hydrocodone bitartrate at a dose of 5mg in 12 healthy Chinese volunteers. Springer-Verlag, 2011 HPLC-MS-MS nationallicence Hydrocodone nationallicence Norhydrocodone nationallicence Hydromorphone nationallicence Hao Guang-Tao Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Chen Yan Bejing Wellso Pharmaceutical Co. Ltd, 100081, Beijing, People's Republic of China aut Dong Rui-Hua Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Qu Heng-Yan Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Liang Yu-Guang Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Li Yuan-Yuan Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Zheng Zhuan-Jie Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Gao Hong-Zhi Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Liu Ze-Yuan Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 567-574 0009-5893 74:7-8<567 2011 74 10337 https://doi.org/10.1007/s10337-011-2118-z text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2118-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hao Guang-Tao Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yan Bejing Wellso Pharmaceutical Co. Ltd, 100081, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Dong Rui-Hua Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Qu Heng-Yan Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liang Yu-Guang Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Yuan-Yuan Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Zheng Zhuan-Jie Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Gao Hong-Zhi Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Ze-Yuan Department of Pharmacology, Affiliated Hospital of the Academy of Military Medical Sciences, 100071, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 567-574 0009-5893 74:7-8<567 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer