caa a22 4500 44580338X CHVBK 20180317145149.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2112-5 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2112-5 HPLC with In-Capillary Optical Fiber Laser-Induced Fluorescence Detection of Picomolar Amounts of Amino Acids by Precolumn Fluorescence Derivatization with Fluorescein Isothiocyanate [Elektronische Daten] [Yunyun He, Lijun Zhao, Hongyan Yuan, Zemin Xu, Ying Tang, Dan Xiao, Martin Choi] In this paper, a fluorescein isothiocyanate (FITC) precolumn derivatization technique in conjunction with an HPLC-in-capillary optical fiber laser-induced fluorescence (HPLC-ICOF-LIF) detection method has been developed for determination of amino acids. The HPLC separation of FITC-labeled amino acids and the ICOF-LIF detection system are studied and optimized. Optimum separation conditions were obtained with a gradient elution program of acetonitrile and phosphate buffer (10mM, pH 6.8). The ICOF-LIF detection system comprises a 530-μm capillary and a 380-μm optical fiber. The analyses of amino acids display excellent linear relationship between peak area and concentration with correlation coefficients greater than 0.999 and the method also provides good repeatability with RSD<3%. The detection limits for FITC-tagged amino acids are very low and the lowest LOD for tyrosine is 51pM. The proposed method has been successfully applied to determination of amino acids in human serum. Our developed HPLC-ICOF-LIF system is cheap, simple, stable, and sensitive which is potentially useful for the formulation analysis and bioanalysis. Springer-Verlag, 2011 Column liquid chromatography nationallicence Amino acids nationallicence FITC nationallicence In-capillary optical fiber laser-induced fluorescence nationallicence He Yunyun College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut Zhao Lijun Test Center for Feed Quality Supervision and Inspection (Chengdu) of the Ministry of Agriculture, 610064, Chengdu, China aut Yuan Hongyan College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut Xu Zemin College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut Tang Ying College of Chemistry and Environmental Engineering, Chongqing University of Arts and Sciences, 402160, Chongqing, China aut Xiao Dan College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut Choi Martin Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, China aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 541-547 0009-5893 74:7-8<541 2011 74 10337 https://doi.org/10.1007/s10337-011-2112-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2112-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- He Yunyun College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Zhao Lijun Test Center for Feed Quality Supervision and Inspection (Chengdu) of the Ministry of Agriculture, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Yuan Hongyan College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Xu Zemin College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Tang Ying College of Chemistry and Environmental Engineering, Chongqing University of Arts and Sciences, 402160, Chongqing, China aut NATIONALLICENCE 700 1- Xiao Dan College of Chemistry and College of Chemical Engineering, Sichuan University, 610064, Chengdu, China aut NATIONALLICENCE 700 1- Choi Martin Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong SAR, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 541-547 0009-5893 74:7-8<541 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer