caa a22 4500 44580341X CHVBK 20180317145149.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2108-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2108-1 Multivariate Optimization and Validation of a CZE Method for the Analysis of Pridinol Mesylate and Meloxicam in Tablets [Elektronische Daten] [Silvana Vignaduzzo, Luciana Vera-Candioti, Patricia Castellano, Héctor Goicoechea, Teodoro Kaufman] A capillary zone electrophoresis method for the simultaneous determination of pridinol mesylate (PRI) and meloxicam (MEL) employing epinastine hydrochloride and piroxicam as internal standards, was developed and optimized employing experimental design and response surface methodologies. The separation was optimally achieved in less than 2min at 30kV in an uncoated fused-silica capillary (41.4cm×75μm I.D.), employing an 18mmolL−1 sodium phosphate buffer solution (pH 5.90) at 25°C. Samples were injected in hydrodynamic mode (50mbar, 5s) and the analytes were spectrophotometrically detected at 200nm. Method robustness was demonstrated by ANOVA of determinations performed under conditions slightly different from the optimum. The method was validated regarding separation selectivity (peak purity factors>0.99), linearity and range (PRI=17.6-31.4mgL−1; MEL=66.5-122.5mgL−1), accuracy (PRI=100.2-101.9%; MEL=98.9-100.7%) and precision. The RSD values obtained were ≤1.3% for injection repeatability and ≤1.9% for intra-day precision. The limits of detection (1.0 and 0.9mgL−1) and quantification (3.3 and 16.5mgL−1) of PRI and MEL, respectively, were also determined. The method was successfully applied to the determination of both drugs in three brands of tablet formulations. No statistically significant differences were observed when these results were compared with those of a RP-HPLC method. Springer-Verlag, 2011 Capillary zone electrophoresis nationallicence Experimental designs nationallicence Meloxicam nationallicence Pridinol nationallicence Vignaduzzo Silvana Pharmaceutical Analysis, Department of Organic Chemistry, Faculty of Pharmaceutical and Biochemical Sciences, National University of Rosario and Institute of Chemistry of Rosario (IQUIR, CONICET-UNR), Suipacha 531, S2002LRK, Rosario, Argentina aut Vera-Candioti Luciana Laboratory of Analytical Development and Chemometrics (LADAQ), Analytical Chemistry I, Faculty of Biochemistry and Biological Sciences, National University of the Litoral, University City, S3000ZAA, Santa Fe, Argentina aut Castellano Patricia Pharmaceutical Analysis, Department of Organic Chemistry, Faculty of Pharmaceutical and Biochemical Sciences, National University of Rosario and Institute of Chemistry of Rosario (IQUIR, CONICET-UNR), Suipacha 531, S2002LRK, Rosario, Argentina aut Goicoechea Héctor Laboratory of Analytical Development and Chemometrics (LADAQ), Analytical Chemistry I, Faculty of Biochemistry and Biological Sciences, National University of the Litoral, University City, S3000ZAA, Santa Fe, Argentina aut Kaufman Teodoro Pharmaceutical Analysis, Department of Organic Chemistry, Faculty of Pharmaceutical and Biochemical Sciences, National University of Rosario and Institute of Chemistry of Rosario (IQUIR, CONICET-UNR), Suipacha 531, S2002LRK, Rosario, Argentina aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 609-617 0009-5893 74:7-8<609 2011 74 10337 https://doi.org/10.1007/s10337-011-2108-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2108-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vignaduzzo Silvana Pharmaceutical Analysis, Department of Organic Chemistry, Faculty of Pharmaceutical and Biochemical Sciences, National University of Rosario and Institute of Chemistry of Rosario (IQUIR, CONICET-UNR), Suipacha 531, S2002LRK, Rosario, Argentina aut NATIONALLICENCE 700 1- Vera-Candioti Luciana Laboratory of Analytical Development and Chemometrics (LADAQ), Analytical Chemistry I, Faculty of Biochemistry and Biological Sciences, National University of the Litoral, University City, S3000ZAA, Santa Fe, Argentina aut NATIONALLICENCE 700 1- Castellano Patricia Pharmaceutical Analysis, Department of Organic Chemistry, Faculty of Pharmaceutical and Biochemical Sciences, National University of Rosario and Institute of Chemistry of Rosario (IQUIR, CONICET-UNR), Suipacha 531, S2002LRK, Rosario, Argentina aut NATIONALLICENCE 700 1- Goicoechea Héctor Laboratory of Analytical Development and Chemometrics (LADAQ), Analytical Chemistry I, Faculty of Biochemistry and Biological Sciences, National University of the Litoral, University City, S3000ZAA, Santa Fe, Argentina aut NATIONALLICENCE 700 1- Kaufman Teodoro Pharmaceutical Analysis, Department of Organic Chemistry, Faculty of Pharmaceutical and Biochemical Sciences, National University of Rosario and Institute of Chemistry of Rosario (IQUIR, CONICET-UNR), Suipacha 531, S2002LRK, Rosario, Argentina aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 609-617 0009-5893 74:7-8<609 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer