caa a22 4500 445803436 CHVBK 20180317145149.0 cr unu---uuuuu 170323e20111001xx s 000 0 eng 10.1007/s10337-011-2109-0 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2109-0 Quantitative Determination of Anti-Inflammatory Columbianetin in Rat Plasma by LC-ESI-MS/MS for Pharmacokinetic Studies after Oral Administration of Duhuo Extract [Elektronische Daten] [Yan-xu Chang, Zi-wei Zhu, Jin Li, Qiu-Hong Zhang, Yan-ru Deng, Liyuan Kang, Bo-li Zhang, Xiu-mei Gao] Specific LC-ESI-MS/MS method or procedure was developed and validated for columbianetin quantification in rat plasma using epicatechin as an internal standard (IS). Chromatographic separation was performed on an Eclipse plus C18 (150×4.6mm, 1.8μm) at a flow rate of 0.300mLmin−1, and water-acetonitrile was used as mobile phase. The calibration curve of the method was linear in the concentration range of 5-1,000ngmL−1. The lower limit of quantification (LLOQ) was 5ngmL−1. The intra- and inter-day precision of the quality control samples was within 15.0%, and the accuracy was within 90.0-110%. The recoveries were more than 90.0% for columbianetin at concentrations of 10, 200 and 1,000ngmL−1, respectively. This method was successfully applied for evaluation of the pharmacokinetics of columbianetin after oral doses of 0.60gkg−1 Angelica pubescence extract in rats. Springer-Verlag, 2011 LC-MS/MS nationallicence Columbianetin nationallicence Angelica pubescence nationallicence Chang Yan-xu Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Zhu Zi-wei Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Li Jin Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Zhang Qiu-Hong Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Deng Yan-ru Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Kang Liyuan Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Zhang Bo-li Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Gao Xiu-mei Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut Chromatographia Springer-Verlag 74/7-8(2011-10-01), 639-643 0009-5893 74:7-8<639 2011 74 10337 https://doi.org/10.1007/s10337-011-2109-0 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2109-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chang Yan-xu Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Zhu Zi-wei Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Li Jin Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Zhang Qiu-Hong Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Deng Yan-ru Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Kang Liyuan Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Zhang Bo-li Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 700 1- Gao Xiu-mei Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 300193, Tianjin, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/7-8(2011-10-01), 639-643 0009-5893 74:7-8<639 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer