caa a22 4500 445803460 CHVBK 20180317145149.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2087-2 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2087-2 Determination of Taspine Using HPLC-MS, and Its Effect on EGFR in A431 and HEK293/EGFR Cells [Elektronische Daten] [Yingzhuan Zhan, Meng Sun, Yinnan Chen, Yanmin Zhang] Taspine was isolated for the first time from Radix et Rhizoma Leonticis. Epidermal growth factor receptor (EGFR) is important in cell growth and differentiation and has become an important target in anti-cancer drug design. In this study, we found taspine could inhibit proliferation of A431 and HEK293/EGFR cells. To investigate whether it could enter the cell or acted on the cell membrane receptor, a cell-based assay was established to determine the concentration of taspine in A431 and HEK293/EGFR cells using high-performance liquid chromatography-mass spectrometry (HPLC-MS). The inhibitory effects of taspine on EGFR expression and mRNA expression were also investigated. The HPLC-MS results showed that taspine decreased in the supernatant liquid, and increased in the cell lysate, which indicated that taspine could enter the cell or act on the cell membrane receptor. Subsequent analysis using a cell-membrane chromatography (CMC) assay showed that taspine could act on EGFR at the cell membrane. In addition, ELISA and reverse transcriptase polymerase chain reaction (RT-PCR) assays showed that taspine inhibited proliferation, EGFR protein, and EGFR mRNA expression in A431 and HEK293/EGFR cells. These results indicated that taspine, with antitumor activity, could interact with the cell, act on EGFR at the cell membrane, and inhibit cell proliferation by down-regulating EGFR protein and mRNA expression. Springer-Verlag, 2011 Liquid chromatography-mass spectrometry nationallicence Taspine nationallicence Inhibition nationallicence EGFR nationallicence Zhan Yingzhuan School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut Sun Meng School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut Chen Yinnan School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut Zhang Yanmin School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 383-389 0009-5893 74:5-6<383 2011 74 10337 https://doi.org/10.1007/s10337-011-2087-2 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2087-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Zhan Yingzhuan School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 700 1- Sun Meng School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yinnan School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yanmin School of Medicine, Xi'an Jiaotong University, No. 76, Yanta Weststreet, #54, 710061, Xi'an, Shaanxi Province, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 383-389 0009-5893 74:5-6<383 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer