caa a22 4500 445803479 CHVBK 20180317145149.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2103-6 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2103-6 Rapid and Simultaneous Study on Drug-Protein Binding of Four Furocoumarins by Hollow Fiber Liquid Phase Microextraction [Elektronische Daten] [Shuang Hu, Yan Zhang, Xiao Bai] Hollow fiber liquid-liquid phase microextraction followed by high-performance liquid chromatography was employed to research the binding of four furocoumarin compounds (psoralen, oxypeucedanin, imperatorin and isoimperatorin) to bovine serum albumin. The results reveal that the percent of drug-protein binding did not rely on the furocoumarins and protein concentrations, and four furocoumarins scarcely competed for protein binding sites. The protein-binding percents of psoralen, oxypeucedanin, imperatorin and isoimperatorin were 55.1±4.5, 14.4±2.4, 44.1±6.7, and 41.3±3.8%, respectively, the numbers of binding sites were 1.2, 1.6, 0.6 and 0.3, and the binding constants were 1,801, 212, 937 and 4,025Lmol−1, respectively. The proposed new approach is simple, rapid, and effective for the study of the interaction between protein and furocoumarin compounds which is either individual or coexistent with the similar ones. Springer-Verlag, 2011 Hollow fiber liquid phase microextraction nationallicence Drug-protein binding nationallicence Furocoumarins nationallicence Simultaneous determination nationallicence Hu Shuang School of Pharmacy, Shanxi Medical University, 030001, Taiyuan, People's Republic of China aut Zhang Yan School of Pharmacy, Shanxi Medical University, 030001, Taiyuan, People's Republic of China aut Bai Xiao School of Pharmacy, Shanxi Medical University, 030001, Taiyuan, People's Republic of China aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 503-506 0009-5893 74:5-6<503 2011 74 10337 https://doi.org/10.1007/s10337-011-2103-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2103-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Shuang School of Pharmacy, Shanxi Medical University, 030001, Taiyuan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yan School of Pharmacy, Shanxi Medical University, 030001, Taiyuan, People's Republic of China aut NATIONALLICENCE 700 1- Bai Xiao School of Pharmacy, Shanxi Medical University, 030001, Taiyuan, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 503-506 0009-5893 74:5-6<503 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer