caa a22 4500 445803517 CHVBK 20180317145149.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2086-3 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2086-3 Fast Screening for Tobacco-Specific N -nitrosamines by CZE Using Dynamically Coated Capillaries [Elektronische Daten] [Jie Liao, Yinjin Pan, Chenchen Li, Dawei Wen, Huwei Liu] In this paper, we propose a rapid capillary zone electrophoretic method with dynamically coated capillaries for separation of and screening for six tobacco-specific nitrosamines, a group of strong carcinogens found only in tobacco products. Dynamic coating of the capillary surface with a polycation coating (CElixir Reagent A) followed by a polyanion coating (CElixir Reagent B; pH2.5) was accomplished by use of a commercially available reagent kit. With this method, six tobacco-specific nitrosamines: N′-nitrosonornicotine, N′-nitrosoanatabine, N′-nitrosoanabasine, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, and 4-(methylnitroamino)-4-(3-pyridyl)-1-butanol were simultaneously separated and determined within 3min, with a satisfactory detection limit of 1.0μgmL−1 for all the tobacco-specific nitrosamines at S/N=3, and a linear relationship between UV absorbance and analyte concentration (R 2>0.98). The repeatability of the method ranged from 0.24 to 0.73% (RSD, n=5) for migration time and 0.25-1.54% (RSD, n=5) for peak area, demonstrating that this capillary electrophoretic method is better than conventional capillary zone electrophoresis. Springer-Verlag, 2011 Capillary zone electrophoresis nationallicence Dynamic coating nationallicence Screening nationallicence Tobacco-specific nitrosamines nationallicence Liao Jie Medical Experiment and Analysis Center, General Hospital of Chinese PLA, 100853, Beijing, China aut Pan Yinjin Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut Li Chenchen Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut Wen Dawei Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut Liu Huwei Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 415-419 0009-5893 74:5-6<415 2011 74 10337 https://doi.org/10.1007/s10337-011-2086-3 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2086-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Liao Jie Medical Experiment and Analysis Center, General Hospital of Chinese PLA, 100853, Beijing, China aut NATIONALLICENCE 700 1- Pan Yinjin Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Li Chenchen Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Wen Dawei Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 700 1- Liu Huwei Key Laboratory of Bioorganic Chemistry and Molecular Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, 100871, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 415-419 0009-5893 74:5-6<415 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer