caa a22 4500 445803533 CHVBK 20180317145149.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2096-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2096-1 GC-MS Coupled with Hollow-Fiber Drop-to-Drop Solvent Microextraction for Determination of Antidepressants Drugs in Human Blood Sample [Elektronische Daten] [Kavita Tapadia, Kamlesh Shrivas, Lata Upadhyay] A simple, rapid and sensitive hollow-fiber with drop-to-drop solvent microextraction (HF-DDSME) combined with gas chromatography-mass spectrometry (GC-MS) has been successfully developed for extraction and determination of antidepressants drugs (AD) in blood sample. The parameters that affect the separation and preconcentration of AD from sample solution were investigated. Calibration curve obtained for three AD were in the range of 100-1,000; 150-1,200; and 80-1,200ngmL−1 for amitriptyline, imipramine, and promethazine, respectively, with correlation coefficient (R 2 ) between 0.990 and 0.997. The limit of detection (LOD) obtained for amitriptyline, imipramine and promethazine was 25, 30 and 18ngmL−1, respectively. The developed method has been successfully applied for the determination of AD concentration in blood sample, and the recoveries for the spiked samples were in the range of 92.3-97.6%. The sample preparation procedure is very simple, effective and virtually solvent-free, and indicated to be a good alternative for the traditional liquid-liquid extraction. Finally, the proposed method was successfully applied for the determination of drug concentration of AD in human blood sample. Springer-Verlag, 2011 Gas chromatography-mass spectrometry nationallicence Hollow-fiber nationallicence Drop-to-drop solvent microextraction nationallicence Antidepressants drugs nationallicence Blood nationallicence Tapadia Kavita Department of Chemistry, National Institute of Technology, 492010, Raipur, Chattisgarh, India aut Shrivas Kamlesh Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, 431-3192, Hamamatsu, Shizuoka, Japan aut Upadhyay Lata Department of Biotechnology, National Institute of Technology, 492010, Raipur, Chattisgarh, India aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 437-442 0009-5893 74:5-6<437 2011 74 10337 https://doi.org/10.1007/s10337-011-2096-1 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2096-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tapadia Kavita Department of Chemistry, National Institute of Technology, 492010, Raipur, Chattisgarh, India aut NATIONALLICENCE 700 1- Shrivas Kamlesh Department of Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, 431-3192, Hamamatsu, Shizuoka, Japan aut NATIONALLICENCE 700 1- Upadhyay Lata Department of Biotechnology, National Institute of Technology, 492010, Raipur, Chattisgarh, India aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 437-442 0009-5893 74:5-6<437 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer