caa a22 4500 445803568 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2085-4 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2085-4 Prostate Cell Membrane Chromatography-LC/MS Method for Screening α1A-Adrenoceptor Antagonists from Lotus Plumule [Elektronische Daten] [Jing Ren, Dongdong Dai, Hui Du, Meng Sun, Sicen Wang] The α1A-adrenoceptor (α1A-AR) plays an important role in drug discovery and development. An online analytical method coupling prostate cell membrane chromatography (CMC) with high-performance liquid chromatography/mass spectrometry (LC/MS) was established to screen α1A-AR antagonists in traditional Chinese medicines (TCMs). The prostate cell membrane stationary phase (CMSP) was prepared by immobilizing the prostate cell membrane onto the surface of the silica carrier. The surface and chromatographic characteristics of the prostate CMSP were studied using tamsulosin as a model molecule. The retained fractions from the prostate CMC were analyzed by transferring them to an LC/MS system through a 10-port switch valve. The active component, which could act on the prostate cell membrane and receptor on it (such as α1A-AR), was determined using a displacement experiment. The results indicated that liensinine, isoliensine, and neferine from Lotus Plumule exhibited similar retention characteristics to the control drug tamsulosin when utilizing the prostate CMC model. This new prostate CMC-LC/MS method is applicable for screening α1A-AR antagonists from TCMs such as Lotus Plumule and could be employed as a drug discovery tool for natural medicinal herbs. Springer-Verlag, 2011 Cell membrane chromatography nationallicence High-performance liquid chromatography/mass spectrometry nationallicence α1A-Adrenoceptor antagonist nationallicence Lotus Plumule nationallicence Prostate nationallicence Ren Jing School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut Dai Dongdong School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut Du Hui School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut Sun Meng School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut Wang Sicen School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 375-381 0009-5893 74:5-6<375 2011 74 10337 https://doi.org/10.1007/s10337-011-2085-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2085-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ren Jing School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 700 1- Dai Dongdong School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 700 1- Du Hui School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 700 1- Sun Meng School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 700 1- Wang Sicen School of Medicine, Xi'an Jiaotong University, 710061, Xi'an, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 375-381 0009-5893 74:5-6<375 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer