caa a22 4500 445803576 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2077-4 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2077-4 Metabonomic Study of Lung Cancer and the Effects of Radiotherapy on Lung Cancer Patients: Analysis of Highly Polar Metabolites by Ultraperformance HILIC Coupled with Q-TOF MS [Elektronische Daten] [Xiaoming Cai, Jun Dong, Lijuan Zou, Xingya Xue, Xiuli Zhang, Xinmiao Liang] In this study, plasma samples were collected from 28 health volunteers and 66 lung cancer patients. Ultraperformance HILIC/Q-TOF MS-based metabonomic techniques were employed to compare the highly polar metabolites (HPMs) in the plasma of patients with lung cancer and those in the plasma of healthy volunteers. High separation efficiency and good repeatability of the HILIC/Q-TOF MS method were observed. Partial least squares discriminant analysis (PLS-DA) of the data from both ESI+ and ESI− mode revealed good classifications between the health volunteer and the lung cancer patients. This result showed that the HPMs from lung cancer patients and healthy controls were significantly different. The correct classification rate of models in both ion modes reached 100%, indicating that the metabonomics method established in this study could predict the plasma samples with high accuracy. Besides, we found 19 ions that show a significant difference in levels between lung cancer patients and healthy controls. Finally, we conducted a primary study of the effect of radiotherapy on HPMs in lung cancer patients. Clear differences among the HPMs of patients in different radiotherapy periods and significant changes in levels of the 19 ions after radiotherapy were noted. This demonstrated that radiotherapy influences the metabolism system of patients. Springer-Verlag, 2011 UPLC/Q-TOF nationallicence Hydrophilic interaction chromatography nationallicence Metabonomics nationallicence Lung cancer nationallicence Highly polar metabolites nationallicence Radiotherapy nationallicence Cai Xiaoming Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut Dong Jun Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut Zou Lijuan Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut Xue Xingya Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut Zhang Xiuli Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut Liang Xinmiao Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 391-398 0009-5893 74:5-6<391 2011 74 10337 https://doi.org/10.1007/s10337-011-2077-4 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2077-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Cai Xiaoming Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Dong Jun Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Zou Lijuan Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Xue Xingya Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Zhang Xiuli Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 700 1- Liang Xinmiao Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 116023, Dalian, China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 391-398 0009-5893 74:5-6<391 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer