caa a22 4500 445803592 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2089-0 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2089-0 Preparation and Performance Research on Glutathione Molecularly Imprinted Polymers [Elektronische Daten] [Yanjun Tong, Yu Xin, Hailin Yang, Ling Zhang, Yuran Zhang, Yi Chen, Xiaole Xia, Wu Wang] Methacrylic acid and ethylene glycol dimethacrylate were respectively used as functional monomer and cross-linker to synthesize molecularly imprinted polymers (MIPs), for adsorption of glutathione (GSH), named GSH-MIPs. Evaluation of various polymers by static experiment indicated the optimum ratio of template to functional monomer was 1:4; the optimal concentration and pH of loading buffer were 5.00-6.50mmol L−1 and 5.0, respectively. By Scatchard analysis, the GSH binding site was found in GSH-MIPs, with K d and Q max of 1.96mmolL−1 and 47.19μmolg−1, respectively. The method validated the extraction of GSH from yeast cells samples with the enrichment rate of 76%. Springer-Verlag, 2011 Molecularly imprinted polymers nationallicence Glutathione nationallicence GSH-MIPs nationallicence Adsorption conditions nationallicence Selectivity nationallicence Efficient extraction nationallicence Tong Yanjun The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Xin Yu The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Yang Hailin The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Zhang Ling The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Zhang Yuran The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Chen Yi The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Xia Xiaole The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Wang Wu The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 443-450 0009-5893 74:5-6<443 2011 74 10337 https://doi.org/10.1007/s10337-011-2089-0 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2089-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tong Yanjun The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Xin Yu The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Yang Hailin The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Ling The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Yuran The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Chen Yi The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Xia Xiaole The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 700 1- Wang Wu The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, 214122, Wuxi, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 443-450 0009-5893 74:5-6<443 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer