caa a22 4500 445803649 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2076-5 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2076-5 Separation of Delphinidin-3- O -sambubioside, Cyanidin-3- O -sambubioside and p -Coumaric Acid from Cranberry by CCC Followed by prep-HPLC Using Robotic CCC Solvent System Selection [Elektronische Daten] [Yun Wei, M. Sardar, Ian Sutherland, Derek Fisher] High-performance counter-current chromatography has been used for the separation of delphinidin-3-O-sambubioside, cyanidin-3-O-sambubioside and p-coumaric acid from crude extract of cranberry. The separation was performed with a two-phase solvent system composed of butanol/0.05% aqueous trifluoroacetic acid/methanol at a volume ratio of 4:5:1. The two-phase solvent system was selected following the determination of partition coefficients (K) in a range of solvent systems using a robotic solvent system selection method. Analytical scale CCC confirmed that this phase system separated the components from a crude cranberry extract (40mg scale) with acceptable purities. Preparative CCC of 400mg of crude yielded 4.2mg of p-coumaric acid at a purity of over 98%, 3.6mg of delphinidin-3-O-sambubioside at a purity of over 97% and 4.5mg of cyanidin-3-O-sambubioside at a purity of 73%, which was further purified by preparative high-performance liquid chromatography to yield 3mg cyanidin-3-O-sambubioside at 95% purity. The identification of delphinidin-3-O-sambubioside, cyanidin-3-O-sambubioside and p-coumaric acid was performed by ESI-MS, 1H-NMR and 13C-NMR spectra. Springer-Verlag, 2011 High-performance counter-current chromatography nationallicence Cranberry nationallicence Delphinidin-3- O -sambubioside nationallicence Cyanidin-3- O -sambubioside nationallicence p -Coumaric acid nationallicence Preparative high-performance liquid chromatography nationallicence Wei Yun State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, China aut Sardar M. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, China aut Sutherland Ian Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge, UB8 3PH, Middlesex, UK aut Fisher Derek Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge, UB8 3PH, Middlesex, UK aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 367-373 0009-5893 74:5-6<367 2011 74 10337 https://doi.org/10.1007/s10337-011-2076-5 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2076-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wei Yun State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, China aut NATIONALLICENCE 700 1- Sardar M. State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, 15 Beisanhuan East Road, Chaoyang District, 100029, Beijing, China aut NATIONALLICENCE 700 1- Sutherland Ian Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge, UB8 3PH, Middlesex, UK aut NATIONALLICENCE 700 1- Fisher Derek Advanced Bioprocessing Centre, Brunel Institute for Bioengineering, Brunel University, Uxbridge, UB8 3PH, Middlesex, UK aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 367-373 0009-5893 74:5-6<367 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer