caa a22 4500 445803665 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2075-6 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2075-6 Single-Step Preparative Extraction of Artemisinin from Artemisia annua by Charcoal Column Chromatography [Elektronische Daten] [Jindi Xu, Jianguang Luo, Lingyi Kong] Activated charcoal column chromatography was successfully applied for preparative extraction of artemisinin with high-purity from Artemisia annua L. in one single run for the first time in this study. The adsorption kinetics study showed that powdery activated charcoal had high adsorption speed for artemisinin. The experimental adsorption data fitted well using the Langmuir adsorption model. The optimal parameters for the extraction of artemisinin were obtained using a column packed with activated charcoal: the loading amount, 1g extract/5g activated charcoal; the elution mode, CH2Cl2-MeOH (2:5, v/v) for 5 BV (bed volume) after the removal of some impurities with CH2Cl2-MeOH (1:10, v/v). With this optimized condition, artemisinin was finally obtained with purity at 95.2% and the recovery at 72.3%. The developed technique would provide a feasible large-scale method for the extraction of artemisinin in pharmaceutical industry. Springer-Verlag, 2011 Charcoal column chromatography nationallicence Artemisinin nationallicence Preparative extraction nationallicence Artemisia annua nationallicence Xu Jindi Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, People's Republic of China aut Luo Jianguang Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, People's Republic of China aut Kong Lingyi Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, People's Republic of China aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 471-475 0009-5893 74:5-6<471 2011 74 10337 https://doi.org/10.1007/s10337-011-2075-6 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2075-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Xu Jindi Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Luo Jianguang Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Kong Lingyi Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, 210009, Nanjing, People's Republic of China aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 471-475 0009-5893 74:5-6<471 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer