caa a22 4500 445803681 CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110901xx s 000 0 eng 10.1007/s10337-011-2092-5 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2092-5 Effects of Temperature and Mobile Phase Condition on Chiral Recognition of Poly( l -phenylalanine) Chiral Stationary Phase [Elektronische Daten] [Kaname Ohyama, Kana Oyamada, Naoya Kishikawa, Mitsuhiro Wada, Yoshihito Ohba, Kenichiro Nakashima, Naotaka Kuroda] Characteristics of the chiral stationary phase with poly(l-phenylalanine) peptide selector, which was in α-helical state, was reported. Since environmental factors affect peptide conformation, the changes in enantioselectivity were examined depending on column temperature and mobile phase conditions (ionic strength, pH, mobile phase composition). Column temperature and pH drastically affected the enantioselectivity. Based on these changes, the relation between chiral recognition and secondary structure of the peptide selector was discussed. The column stability during sequential analysis under different separation conditions was also evaluated. Springer-Verlag, 2011 Chiral stationary phase nationallicence Column temperature nationallicence Helical conformation nationallicence l -Phenylalanine nationallicence Mobile phase condition nationallicence Ohyama Kaname Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut Oyamada Kana Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut Kishikawa Naoya Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut Wada Mitsuhiro Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan aut Ohba Yoshihito Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagaski, Japan aut Nakashima Kenichiro Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan aut Kuroda Naotaka Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut Chromatographia Springer-Verlag 74/5-6(2011-09-01), 467-470 0009-5893 74:5-6<467 2011 74 10337 https://doi.org/10.1007/s10337-011-2092-5 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2092-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ohyama Kaname Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut NATIONALLICENCE 700 1- Oyamada Kana Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut NATIONALLICENCE 700 1- Kishikawa Naoya Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut NATIONALLICENCE 700 1- Wada Mitsuhiro Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan aut NATIONALLICENCE 700 1- Ohba Yoshihito Faculty of Pharmaceutical Sciences, Nagasaki International University, Nagaski, Japan aut NATIONALLICENCE 700 1- Nakashima Kenichiro Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan aut NATIONALLICENCE 700 1- Kuroda Naotaka Department of Environmental and Pharmaceutical Sciences, Course of Pharmaceutical Sciences, Graduate School of Biomedical Sciences, Nagasaki University, 1-14 Bunkyo-machi, 852-8521, Nagasaki, Japan aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/5-6(2011-09-01), 467-470 0009-5893 74:5-6<467 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer