caa a22 4500 44580372X CHVBK 20180317145150.0 cr unu---uuuuu 170323e20110701xx s 000 0 eng 10.1007/s10337-011-2019-1 doi (NATIONALLICENCE)springer-10.1007/s10337-011-2019-1 MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix [Elektronische Daten] [Joanna Znaleziona, Vítězslav Maier, Jan Petr, Jan Chrastina, Juraj Ševčík] The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7min using 50mM sodium borate pH 9.0 with the addition of 50mM sodium dodecylsulfate at +20kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150mM NaCl was applied. The limit of detection after the preconcentration step for nilutamide was 26μgL−1. Springer-Verlag, 2011 Micellar electrokinetic chromatography nationallicence High salt sample stacking nationallicence Sweeping nationallicence Serum nationallicence Nilutamide nationallicence Znaleziona Joanna Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut Maier Vítězslav Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut Petr Jan Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut Chrastina Jan Faculty of Health Sciences, Palacký University in Olomouc, Tr. Svobody 8, 77146, Olomouc, Czech Republic aut Ševčík Juraj Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut Chromatographia Springer-Verlag 74/1-2(2011-07-01), 151-155 0009-5893 74:1-2<151 2011 74 10337 https://doi.org/10.1007/s10337-011-2019-1 text/html Onlinezugriff via DOI 1 brief-communication jats NATIONALLICENCE 856 40 https://doi.org/10.1007/s10337-011-2019-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Znaleziona Joanna Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Maier Vítězslav Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Petr Jan Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Chrastina Jan Faculty of Health Sciences, Palacký University in Olomouc, Tr. Svobody 8, 77146, Olomouc, Czech Republic aut NATIONALLICENCE 700 1- Ševčík Juraj Department of Analytical Chemistry, Faculty of Science, Palacký University in Olomouc, 17. Listopadu 12, 77146, Olomouc, Czech Republic aut NATIONALLICENCE 773 0- Chromatographia Springer-Verlag 74/1-2(2011-07-01), 151-155 0009-5893 74:1-2<151 2011 74 10337 Metadata rights reserved Springer special CC-BY-NC licence nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-springer